ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (12), pp. 001-006, December, 2018. © International Scholars Journals
Full Length Research Paper
A magnetic microparticle-based immunoassay for hepatitis C virus NS3 antigen
Li Xie1*, Yue-ping Guang2, Fang Liu1, Hong-bo Shi1, Li Yan1 and Jin-li Lou1
1Beijing You’an Hospital, Capital Medical University, Beijing 100069, China.
2University of Science and Technology Beijing, Beijing 100083, China.
Accepted 04 December, 2018
Abstract
In order to detect Hepatitis C virus NS3 antigen (HCAg-NS3) in human serum, we developed a magnetic microparticle (MMP) based immunoassay by using biotin-streptavidin amplification, MMP in combination with immunoassay. Results showed that the HCV NS3 monoclonal antibody had a high immobility rate (87.1%) on the surface of MMPs; the assay procedure required smaller volumes of reagents (only 20 l of immune magnetic bead); the detection could be completed within 30 min; this method could detect 10 pg/ml HCAg-NS3 (sensitivity: 5.2 pg/ml) and had favorable stability; the mean coefficient of variances (CVs) of intra-assay and inter-assay were 5.35 and 7.15%, respectively. The results of this method were also related to those in HCV RNA detection with a linear correlation coefficient of 0.570 (P < 0.01). These findings demonstrate this assay which has high specificity, sensitivity, precision and stability may be promising in clinical application.
Key words: Hepatitis C virus NS3 antigen, magnetic microparticle, biotin-streptavidin sytem (ABS), magnetic immunoassay.