Advanced Journal of Microbiology Research

ISSN 2736-1756

Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (6), pp. 001-006, June, 2019. © International Scholars Journals 

Full Length Research Paper

In vitro evaluation of antimicrobial activity of methanolic extract from selected species of Cephalopods on clinical isolates

Pasiyappazham Ramasamy1, Namasivayam Subhapradha1, Alagiri Srinivasan2, Vairamani Shanmugam1, Jayalakshmi Krishnamoorthy1 and Annaian Shanmugam1*

1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University Parangipettai – 608 502, India.

2Department of Biophysics, All India Institute of Medical Science, New Delhi – 110029, India.

Accepted 10 March, 2019

Abstract

Bioactive substances from marine biota have been found useful as special tools in pharmacological and biomedical research. In the present study, the in vitro antimicrobial activity of crude methanolic extracts of six species of cephalopods (Sepia kobiensis, Sepiella inermis, Sepioteuthis lessoniana, Octopus aegina, Octopus aerolatus, Octopus dollfusi) from Cuddalore (Southeast coast of India) was studied. The antimicrobial activity was screened against 10 species of clinically isolated human pathogenic bacteria namely Vibrio chlolerae, Pseudomonas aeruginosa, Klebsiella pnemoniae, Vibrio alginolyticus, Staphylococcus aureus, Vibrio parehaemolyticus, Streptococcus sp., Streptococcus pnemoniae, Salmonella sp. and Escherichia coli and five fungal strains such as Alternaria alternata, Candida tropicalis, Pencillium italicum, Fusarium equisetii and Candida albicans. Different concentrations such as 25, 50, 75 and 100% were prepared and tested against the microbial strains for their inhibitory activities, using the disc diffusion method. The minimum inhibitory concentration (MIC) of methanolic extract of cephalopods ranged from 60 to 100 mg/ml. The results were discussed in the light of positive and negative control apart from the concentrations tested.

Keywords:  Antimicrobial activity, cephalopods, minimum inhibitory concentration (MIC), pathogenic microorganisms.

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Alagiri Srinivasan on Google Scholar Alagiri Srinivasan on Pubmed Jayalakshmi Krishnamoorthy and Annaian Shanmugam* on Google Scholar Jayalakshmi Krishnamoorthy and Annaian Shanmugam* on Pubmed Namasivayam Subhapradha on Google Scholar Namasivayam Subhapradha on Pubmed Vairamani Shanmugam on Google Scholar Vairamani Shanmugam on Pubmed Pasiyappazham Ramasamy on Google Scholar Pasiyappazham Ramasamy on Pubmed