Advanced Journal of Microbiology Research

ISSN 2736-1756

Advanced Journal of Microbiology Research Vol. 2003

Available online at http://internationalscholarsjournals.org/journal/ajmr

© 2003 International Scholars Journals

Cellulase Production by Aspergillus flavus Linn Isolate NSPR 101 fermented in sawdust, bagasse and corncob

OJUMU, Tunde Victor1*, SOLOMON, Bamidele Ogbe2,e, BETIKU, Eriola2, , LAYOKUN, Stephen Kolawole 2, and AMIGUN, Bamikole3

1Engineering Materials Development Institute, P.M.B 611, Akure Nigeria.

2Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Nigeria.

3Department of Food Science and Technology, Federal Polytechnic Ado-Ekiti, Ekiti State, Nigeria.

Accepted 23 May 2003

Abstract

Bagasse, corncob and sawdust were used as lignocellulosic substrates for the production of cellulase enzyme using Aspergillus flavus after ballmilling and pretreatment with caustic soda. From the fermentation studies, sawdust gave the best result with an enzyme activity value of 0.0743IU/ml while bagasse and corncob gave 0.0573IU/ml and 0.0502IU/ml respectively. The three lignocellulosics gave their maximum enzyme activities at about the twelfth hour of cultivation, suggesting that the 12th hour is the optimum time when the enzyme may be harvested.

Key words: Aspergillus flavus, cellulase activity, lignocellulosics.