ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Isolation and characterization of micro satellites in Bambusa arundinacea and cross species amplification in other bamboos
Sumitra Nayak and Gyana Ranjan Rout*
Plant Biotechnology Division, Regional Plant Resource Center, Nayapalli, Bhubaneswar –751 015, Orissa, India
Accepted January 15, 2005
Abstract
Isolation and characterization of microsatellites was analyzed in Bambusa arundinacea and cross species amplification in other bamboos. Six microsatellites, three polymorphic and three monomorphic, were characterized in a bamboo species, Bambusa aruninacea belonging to the family Poaceae. The numbers of alleles per locus ranges form 2 to 6. Allelic diversity ranges from 0.128 to 0.789. Polymorphic Information Content (PIC) values for two loci were > 0.3, as an indicator of polymorphic allele. Cross species amplification has been tested in other 18 bamboo species. Monomorphic simple sequence repeats (SSRs) have been found to be cross amplified in most of the tested species while polymorphic ones in only three to four species. Utilization of the SSR loci in genetic diversity study of B. arundinacea and other cross amplified bamboo species is discussed.
Key words: Bambusa arundinacea, microsatellite marker, cross species amplification, population study, species identification.