ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2011
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2011 International Scholars Journals
Full Length Research Paper
Optimization of cellulase production by Aspergillus niger and Tricoderma viride using sugar cane waste
Ali A. Juwaied, Ahmed Abdulamier Hussain Al-amiery*, Zahraa Abdumuniem and Umar Anaam
Department of applied science, Biotechnology division, University of Technology, Baghdad, Iraq.
Accepted 1 February, 2011
Abstract
Sugar cane waste was used as substrates for the production of cellulase enzyme using Aspergillus niger and Tricoderm aviride. From the fermentation studies, sugar cane waste gave the best result with an enzyme activity value of (using A. niger and T. aviride) 2.7 and 2.6 IU/ml respectively. It gave the maximum enzyme activity at about the 10 days of cultivation, suggesting that is the optimum time when the enzyme may be harvested. The fermentation experiments were performed in solid stat fermentation (SSF). Incubation time and initial pH of fermentation medium watts optimized with simultaneous mixed culture. It was revealed that the sugar cane waste at pH = 4.5 and 40°C Aspergillus niger (37°C for T. viride) was the best source of carbon for the enhanced production of cellulase in the compatible culture experiments. Based on the reported results, it may be concluded that sugar cane waste can be a potential substrate for production of cellulase, incorporation of culturing A. niger or T. viride. The aim of this work is to produce cellulase from sugar cane waste and reduce the pollution.
Key words: Aspergillus niger, cellulase, culture, sugar cane waste.