ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Characterization of Clostridium perfringens field isolates, implicated in necrotic entritis outbreaks on private broiler farms in Cairo, by multiplex PCR
Effat, M. M1*; Abdallah, Y. A.2, Soheir, M. F.1, and Rady, M. M.3
1Microbiology and Immunology dept, National research centre, Giza, Egypt.
2Serum and Vaccine Research Institute, Abbassia, Egypt.
3Poultry Diseases dept, National Research centre, Giza, Egypt.
Accepted 25 July 2012
Abstract
During the winter of 2006, outbreaks of severe entritis affected many broiler farms in the AL-Fayoum governorate, south of Cairo, Egypt. To identify the causative agent(s), bacterial isolates (14) from the diseased chickens were characterized phenotypically and biochemically. All isolates exhibited characteristics of Clostridium perfringens. Subsequently, molecular typing of the bacterial isolates was performed by multiplex PCR using four sets of primers specific for the genes encoding the C. perfringens , , and toxins, respectively. A single amplicon, corresponding in size to the alpha ( ) toxin-encoding gene (approximately 402 bp), was amplified from all the bacterial isolates. It was therefore concluded that only C. perfringens type A was responsible for the disease outbreaks.
Key words: Clostridium perfringens- necrotic entritis – broiler chicken – multiplex PCR.