ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2013
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2013 International Scholars Journals
Full Length Research Paper
Isolation and partial characterization of bacteriocin produced from Lactobacillus plantarum
V. Karthikeyan* and S. W. Santosh
Department of biotechnology, School of Bioengineering, SRM University, Kattankulathur-603203, Tamilnadu, India.
Accepted 19 February, 2013
Abstract
Bacteriocin producing Lactobacillus plantarum strain isolated from marine shrimp (Penaeus monodon) gut, showed broad range of antibacterial activity against some major food born pathogens. Maximum bacteriocin production was observed at 50°C, pH 4 and 0.9% sodium chloride solution. The bacteriocin has purified by ammonium sulphate precipitate and ion exchange (DEAE cellulose) chromatography. Biochemically it was pure protein moiety and the molecular weight was 2.5 KDa based on the plasmid curing experiment, suggesting that the bacteriocin was a plasmid encoded protein molecule. The study revealed the possibility of using bacteriocin as a food preservative and the L. plantarum strain as probiotic.
Key words: Antagonistic activity, bacteriocin, chromatography, Lactobacillus plantarum.