ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 15 (1), pp. 001-008, January, 2021. © International Scholars Journals
Full Length Research Paper
Assessment of Paramecium tetraurelia growth in a new synthetic culture medium
AZZOUZ Zoubir1,2*, BERREBBAH Houria2 and DJEBAR Mohamed Reda2
1Department of marine Sciences, Faculty of Sciences, Badji Mokhtar University of Annaba, Algeria.
2Laboratory of Cellular Toxicology, Department of Biology, Faculty of Sciences, Badji Mokhtar University of Annaba, 23000, B.P. 12. Algeria.
Accepted 22 August, 2020
Abstract
For using an organism for ecotoxicological studies, it is important to understand its model of growth under some physicochemical parameters that influence with a direct manner its development. The knowledge of the optimal parameters for the paramecia development leads to realize a model of growth with clear stages. For that, we tried to study the influence of temperature, pH, nutrient density in the culture medium and pollution on the growth of Paramecium tetraurelia. Obtained results showed that paramecia prefer definite conditions. However, the growth was better in temperatures included between 25 and 30°C, with preference to increased temperatures and a slightly acidic pH. The culture medium was prepared from the mixture of several vegetables which the rates were studied depending to the growth. The combined effects of azoxystrobin and cyproconazole on the growth kinetics were studied. Results showed that treatment affected the population growth, the generation time and the velocity of generation.
Key words: Paramecium tetraurelia, growth kinetics, physicochemical parameters, azoxystrobin, cyproconazole.