ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-004, July, 2018. © International Scholars Journals
Full Length Research Paper
Phytochemical and antimicrobial screening of methanol extract of Heliotropium indicum leaf
Osungunna, M. O.1* and Adedeji, K. A.2
1Department of Pharmaceutics, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Ogun State, Nigeria.
Accepted 24 May, 2018
Abstract
Methanol extract of the leaf of Heliotropium indicum was evaluated for its antimicrobial activity against five bacterial isolates comprising of four Gram-negative namely: Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp. and Proteus mirabilis; and one Gram positive, Staphylococcus aureus at 6.25, 12.5, 25, 50, 100 and 200 mg/ml including phytochemical analysis. While both S. aureus and Klebsiella spp. were inhibited at 50, 100 and 200 mg/ml with minimum inhibitory concentration (MIC) of 3 mg/ml; P. aeruginosa and P. mirabilis with MIC of 10 mg/ml were inhibited at 100 mg/ml and 200 mg/ml. E. coli with MIC of 20 mg/ml was inhibited only at 200 mg/ml concentration of the extract. Phytochemical analysis revealed the presence of plant metabolites as alkaloids, saponin and tannins. While activity-directed assay is advocated, the plant promises to be of tremendous assistance in the treatment of infections with which bacterial isolates used for the study are associated.
Key words: Phytochemical, antimicrobial, Heliotropium indicum alkaloids, saponin, tannin.