Frontiers of Agriculture and Food Technology

Frontiers of Agriculture and Food Technology ISSN 2736-1624 Vol. 10 (7), pp. 001-003, July, 2020. © International Scholars Journals

Full Length Research Paper

Bioremediation of oil-polluted soil by Lentinus subnudus, a Nigerian white-rot fungus

Adenipekun, C. O.* and Fasidi, I. O.

Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria.

Accepted 21 June, 2020

Abstract

Inspite of the realization and studies on the use of microorganisms in degrading hydrocarbons there has been very little work on indigenous white- rot fungi in Nigeria, a leading oil – producing country. The ability of Lentinus subnudus to mineralize soil contaminated with various concentrations of crude oil was tested. Organic matter and carbon were higher than the control at all concentrations of crude oil contamination in soils inoculated with L. subnudus for 3 months. Nutrient contents were generally higher after 6 months of incubations except potassium levels which were not significantly different from the control. As for the total petroleum hydrocarbon (TPH) in crude – oil contaminated soils; the highest rate of biodegradation was at 20% concentration after 3 months and 40% after 6 months of incubation.

Key words: bioremediation, crude oil, Total Petroleum Hydrocarbon oil-polluted soil, Lentinus subnudus.

Frontiers of Agriculture and Food Technology ISSN 2736-1624 Vol. 10 (7), pp. 001-005, July, 2020. © International Scholars Journals

Key words: Chillies, aflatoxin B₁, cold storage.

Frontiers of Agriculture and Food Technology ISSN 2736-1624 Vol. 10 (7), pp. 001-006, July, 2020. © International Scholars Journals

Frontiers of Agriculture and Food Technology ISSN 2736-1624 Vol. 10 (6), pp. 001-005, June, 2020. © International Scholars Journals

Full Length Research Paper

Bacteria and fungi isolated from housefly (Musca domestica L.) larvae

Banjo, A.D., Lawal, O.A.*and Adeduji, O.O.

 Department of Biological Sciences, Olabisi Onabanjo University, Ago-Iwoye, P.M.B. 2002, Ogun State, Nigeria.

Accepted 15 March, 2020

Abstract

Housefly larvae were cultured on fresh fish and collected for the isolation and identification of microorganisms associated with them. The microbes were cultured from both the gut and body surface of the maggot on nutrient agar (for bacteria) and potato dextrose agar (for fungi) and incubated at about 37°C for 48 h before observations. A variety of microorganisms, which includes the pathogenic Staphylococcus aureus, Pseudomonas aerruginosa, Aspergillus tamarii and Bacullus cereus were found. Also, nonpathogenic microbes werer recovered including Bacillus subtilis and Stroplococcus faecalis.

Key words: Microorganism, isolation, housefly larvae.

Frontiers of Agriculture and Food Technology ISSN 2736-1624 Vol. 10 (6), pp. 001-004, June, 2020. © International Scholars Journals

Full Length Research Paper

Production dynamics of extracellular protease from Bacillus species

Folasade M. OLAJUYIGBE* and Joshua O. AJELE

Department of Biochemistry, School of Sciences, Federal University of Technology, Akure, Nigeria.

Accepted 17 April, 2020

Abstract

Screening and isolation of proteolytic bacteria were carried out from soil samples of Ikogosi warm spring (SW, Nigeria) . Eighteen isolates were positive on skim milk agar (10%) of which fifteen produced protease in culture broth. Three isolates, identified as Bacillus macerans IKBM-11, B. licheniformis IKBL-17 and B. subtilis IKBS-10, were selected for further study. These Bacillus species could grow up to 65°C within a broad pH range of 5 to 10 with an optimal growth temperature and pH at 60°C and 8.0, respectively. For the three Bacillus species, protease production occurred between 37°C and 65°C and pH 5 to 10. Maximum growth and maximum enzyme production was observed at 48 h when grown in 50 ml medium (pH 8.0) under shaking condition at 60°C. The results showed that Bacillus species under study are good producers of extracellular protease at high temperature. This might be an indication that proteases produced would be thermostable.

Key words: Protease, proteolytic bacteria, Bacillus macerans, Bacillus licheniformis, Bacillus subtilis.

Frontiers of Agriculture and Food Technology ISSN 2736-1624 Vol. 10 (6), pp. 001-004, June, 2020. © International Scholars Journals

Short Communication

Isozyme diversity in Cassia auriculata L.

R. Siva^1,2* and K.V. Krishnamurthy²

¹The Institutes for Applied Research, Ben Gurion University of the Negev, Beer Sheva, Israel.

²Department of Plant Science, Bharathidasan University, Tiruchirapplli, Tamil Nadu, India.

Accepted 16 April, 2020

Abstract

Cassia auriculata is considered to be one of the important dye yielding and medicinal plants in India. In the present study seeds from fourteen different localities were collected all over India and nine enzymes were screened by native polyacrylamide gel electrophoresis (PAGE) technique and thirty-four putative loci were totally detected. Cluster and factor analyses indicated that there are two major distinct groups or clusters, and thus, seeds collected from a few different localities are enough to capture the genetic variation held by this species. Also isozyme analysis is a reliable, efficient and effective marker technology for determining genetic variations in C. auriculata.

Key words: Genetic diversity, isozyme, Cassia auriculata, dye.