International Journal of Biochemistry and Biotechnology

International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 13 (6), pp. 001-003, June, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Short Communication

Evaluation of various diagnostic techniques for detecting Entamoeba histolytica in emergency department patients with diarrhea

Ozlem Aycan Kaya¹, Hakan Oguzturk², Muhammet Gokhan Turtay², U. Nilgun Daldal³

¹Çö nük District. Necip Fazil Kisakürek Street/ 4. Ihlamur Building, 2/6- Malatya, Turkey.
²Department of Emergency Medicine, Faculty of Medicine, Inonu University, 44280 Malatya, Turkey.
³Department of Medical Parasitology, Faculty of Medicine, Inonu University, 44280 Malatya, Turkey.

Accepted 18 October, 2023

Abstract

Antigen screening were conducted to stool samples from 60 patients admitted to our emergency department with diarrhea complaint between June 2009 and October 2009 by the methods of direct microscopic examination, trichrome staining, ELISA (Enzyme-linked immunosorbent assay), respectively. As a result of examination of total 60 samples with direct microscopic, trichrome staining and ELISA method, it was detected positive in 7(11.3%), 6(9.7%) and 8(12.9%) samples, respectively. The presence of Entamoeba histolytica has been accepted exactly in the samples in which ELISA test results were positive and necessary treatment of patients has been started immediately. Due to precise pathogen protozoan discrimination has not been performed with the direct microscopic examination, it was emphasized that unnecessary drug therapy would be prevented as a result of detection of presence of E. histolytica specific antigen by ELISA in the samples sent to the laboratory with the diagnosis of amoebiasis by concerned physician.

Key words: Entamoeba histolytica, diarrhea, diagnosis.
 

International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 13 (5), pp. 001-003, May, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Short Communication

Unveiling the Antibacterial and Antifungal Potential of Xylaria Polymorpha: A Novel Natural Source of Antimicrobial Agents

Nurcihan Hacioglu¹, Ilgaz Akata² and Basaran Dulger¹*

¹Department of Biology, Faculty of Science and Arts, Canakkale Onsekiz Mart University, 17100 Canakkale, Turkey.
²Department of Biology, Faculty of Science, Ankara University, 06100 Ankara, Turkey.

Accepted 1 November, 2023

Abstract

The ethanolic extracts obtained from the macrofungus Xylaria polymorpha (Pers.) Grev. (Ascomycetes) have been investigated for their antimicrobial activity. Growth inhibition using agar disc diffusion assays was determined against Escherichia coli ATCC 11230, S. aureus ATCC 6538P, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403, Geotrichum capitatum ATCC 28576, Debaryomyces hansenii DSM 70238, Kluyveromyces fragilis ATCC 8608 and Cryptococcus neoformans ATCC 90112. As a result of study, we have found that X. polymorpha revealed antimicrobial activity against all tested microorganisms. Especially, G. capitatum is more susceptible to the extracts.

Key words: Xylaria polymorpha, antimicrobial activity.

International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 13 (4), pp. 001-003, April, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Short Communication

A comparative analysis of the pharmacological effectiveness of Berberis lycium and penicillin g

Ihtisham Bukhari¹*, Mukhtiar Hassan¹, Fida M. Abbasi¹, Ghulam Mujtaba², Nasir Mahmood³, Noshin¹, Anees Fatima¹, Muhammad Afzal¹, Mujaddad Ur Rehman¹, Farzana Perveen¹ and M. Tariq Khan¹

¹Hazara University, Garden Campus Mansehra Pakistan.
²PCR labs INMOL Lahore, Pakistan.
³Department of Allied Health sciences, University of Health sciences Lahore, Pakistan.

Accepted 1 November, 2023

Abstract

The importance of medicinal plants in traditional healthcare practices provides opportunity to new areas of research. However, information on the uses of plants for medicine is lacking from many interior areas of District Mansehra. Keeping this in view, the present study was initiated. This study attempts to shade lights on the comparative pharmacological efficacy of Berberis lycium (medicinal plant) and drug penicillin G. The pharmacological activities of both the B. lycium and penicillin G were determined by disc diffusion method with incubation period of 24 to 48 h at 37°C. It was observed that, B. lycium showed excellent activity against the tested microorganism, Escherichia coli and Proteus (80 to 100%) and good activities against the tested microorganisms pseudomonas and staphylococcus (60 to 70%). While penicillin G showed excellent activity against all tested microorganisms. It is recommended that, B. lycium can be used for the treatment of diseases caused by tested microorganisms because of its excellent and good activity. It is also observed that, B. lycium do not have any adverse effect on those people who have this in long practice.

Key words: Berberis lycium, penicillin G, disc diffusion, pharmacological activity.
 

International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 13 (3), pp. 001-005, March, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Serum levels of complement C1, C3, and C4 in leishmaniasis cases in Turkey

Limoncu M. E.¹*, anlida    T.², Balcio lu I. C.³, Akçali S.², Özensoy S.⁴ and Özbel Y.⁴

¹Department of Microbiology, Vocational School of Health Services, Celal Bayar University, Manisa, Turkey.
²Department of Microbiology, Celal Bayar University School of Medicine, Manisa, Turkey.
³Department of Parasitology, Celal Bayar University School of Medicine, Manisa, Turkey.
⁴Department of Parasitology, Ege University School of Medicine, zmir, Turkey.

Accepted 28 October, 2023

Abstract

Leishmaniasis is still a problem for many countries including developed ones. The subgroups of the serum complements have significant roles on the onset of the infection. The aim of this study was to determine the serum complement (C1, C3, C4) levels of cases with definite diagnosis of leishmaniasis and compare them with healthy controls. The study group included 43 visceral leishmaniasis (VL) and 13 cutaneous leishmaniasis (CL) cases. Two control groups were formed for the study. The first control group included 50 individuals of the same age group having no health complaints, admitted for routine control and found to be serologically negative. The second control group included 28 individuals, consistent with the diagnostic criteria of VL. The serum complement levels of C1, C3 and C4 were measured by the nephelometric method. The average levels of C1 were found to be high in VL cases (78.98%) and low in CL (41.69%) group, which was statistically significant (p=0.011). In addition, the average levels of C3 were high in CL group (96.08%) and low in VL group (62.00%), which was also statistically significant (p=0.010) . C4 levels were found to be high in the control group, while similar in VL and CL groups. C1, C3, C4 levels were found to be lower in the first control group of healthy individuals. The levels of the subgroups of complement system show statistically significant in both VL and CL cases, which suggested that they could be useful in verifying the results of the serological examinations.

Key words: Leishmaniasis, complement levels, C1, C3, C4.

International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 13 (2), pp. 001-006, February, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Influence of glomus mosseae arbuscular mycorrhizal fungi on leaf development traits of paeonia suffruticosa under salinity conditions

Guo Shaoxia^1,2* Han Tingting² and Liu Runjin¹

¹Institute of Mycorrhizal Biotechnology, Qingdao Agricultural University, Qingdao Shandong Province 266109, China.
²College of Garden and Horticulture, Qingdao Agricultural University, Qingdao Shandong Province 266109, China.

Accepted 28 October, 2023

Abstract

The effects of Arbuscular mycorrhizal (AM) fungi on the characteristics of leaf development of Paeonia suffruticosa at different levels of salt stress (0, 8, 16 and 24%) were studied. Potted ‘Feng Dan’ seedlings were inoculated with Glomus mosseae, and the non-inoculated was used as the control. The results showed that under salt stress leaf relative water content, sclerophyllous index of leaves and leaf succulence level of P. suffruticosa seedlings inoculated with G. mosseae were significantly higher than those of non-inoculated seedling, and specific leaf area was significantly lower than that of the control. Leaf water loss rate of the non-inoculated peony was faster, the percentage of water loss in the total amount of water each time point is the highest. These results suggest that G. mosseae may play an important role in the leaf traits and enhanced salt tolerance of tree peony seedlings.

Key words: Arbuscular mycorrhizal fungi, characteristics of leaf development, Paeonia suffruticosa, salt stress.