African Journal of Parasitology Research

African Journal of Parasitology Research  ISSN 2343-6549 Vol. 4 (9), pp. 257-263, November, 2017. © International Scholars Journals

Full Length Research Paper

Intestinal parasitic infections among prison inmates at theMACA – Maison-d’Arrêt-et de Correction d’Abidjan, Côte d’Ivoire

Kiki-Barro PCM*¹,  Angora EK¹, Konaté A¹, Kassi FK^1,2, Vanga-Bosson H¹, Bedia-Tanoh AV¹, Djohan V¹, Yavo W¹, Menan EIH^1,2

¹Parasitology and Mycology Department, Faculty of Pharmacy, 01 BP V 34, Abidjan, Côte d’Ivoire.

²Center for the diagnosis and research on AIDS and other infectious diseases, Abidjan BP V 3 Côte d’Ivoire.

Corresponding author. E-mail: [email protected]. Tél : +22507584947

Accepted 05 July, 2017

Abstract

Intestinal parasitic infection epidemiology in the Ivorian prisons is little documented. The purpose of this study was to estimate the prevalence of intestinal parasites carriage at MACA. This cross-sectional study (January to July 2013) was carried out among 300 inmates aged 16 – 84 years. Single stool samples were collected and analyzed using the direct examination, the Kato-Katz and Baermann techniques. The overall prevalence of parasitic intestinal infections prevalence was 69.3%, (95%CI = 63.9 – 74.4). Regarding the intestinal protozoa, amoebas Entamœba coli-(54.0%) and Endolimax nana (6.0%) were the most encountered. Giardia intestinalis (1.4%) was the only flagellated found. Among helminths, hookworms (6.0%) was the predominant parasites, followed by Ascaris lumbricoides (3.0%), Trichuristrichiura, (3.0%) and Strongyloïdesstercoralis, (0.7%). Prisoners under 45 years old (OR=2.2, 95%CI=1.2-4.3), illiterate inmates (OR=5.8, 95%CI=3.1-10.5), duration of incarceration (OR=5.6, 95%CI=3.2-9.9) were significant risk factors of intestinal parasitic infections among prisoners in MACA. This study urges the setting up of a program to fight against the intestinal parasitic infections in the Ivorian prisons.

Keywords: Intestinal parasitic infections, prisons, Abidjan, Côte d’Ivoire.

African Journal of Parasitology Research ISSN 2343-6549 Vol. 4 (10), pp. 264-270, December, 2017. © International Scholars Journals

Full Length Research Paper

Schistosoma mansoni infection and hematological profile in an endemic foci in Western Burkina Faso

Hermann Sorgho¹*, Ollo Da², Toussaint Rouamba¹, Boubacar Savadogo³, Halidou Tinto¹, Jean-Bosco Ouedraogo⁴

¹Institut de Recherche en Sciences de la Santé - Unité de Recherche Clinique de Nanoro, 11 BP 218 Ouaga CMS 11, Burkina Faso.

²Centre Hospitalier Universitaire SouroSanou, 01 BP 676, Bobo-Dioulasso 01, Burkina Faso.

³Institut de Recherche en Sciences de la Santé – Département BIOMED-SP, 03 BP 7192 Ouagadougou, Burkina Faso.

⁴Institut de Recherche en Sciences de la Santé, Direction Régionale de l’Ouest, 01 BP 545, Bobo-Dioulasso 01, Burkina Faso.

Accepted 29 October, 2017

*Corresponding author: E-mail: [email protected]. Tel : +226 70117109

Abstract

Persistence of light infections of Schistosoma mansoni is common among individuals living in endemic settings despite the mass drug treatment with praziquantel. Therefore, it is of interest to understand the impact of S. mansoni on general health indicators such as hematological profile. In the present study, we investigated the impact of S. mansoni infection on complete blood counting an endemic foci in Western Burkina Faso. The results showed among schistosomiasis endemic population, in addition to anemia, participants having poor hematological profile had higher risk of being infected by S. mansoni (OR = 1.71; 95% CI 1.27 – 2.3; P = 0.0003). Conversely, S. mansoni positive subjects had a higher risk of harboring poor hematological profile (OR = 1.49; 95% CI 1.06 – 2.10; P = 0.023) when adjusted by age, gender and water contact. A longer follow-up would allow a better understanding of the long-term impact of schistosomiasis control program on important health indicators such as hematological profile.

Keywords: Schistosomiasis mansoni, hematological profile, ascending hierarchical clustering analysis.

African Journal of Parasitology Research ISSN 2343-6549 Vol. 4 (9), pp. 249-256, November, 2017. © International Scholars Journals

Full Length Research Paper

Propagation in culture of Plasmodium falciparum gametocytes clinical isolates and antiplasmodial activity of two medicinal plant extracts on parasitic growth

Beourou S¹*,Okou OC², Tuo K¹, Kamenan KCD², Gnondjui AA¹, Kouakou L¹, Attemene DSD³,   Penali L¹, Ronan Jambou^1,5, Djaman AJ^1,4,6

¹Laboratory of Malariology, Department of Parasitology and Mycology, Institut Pasteur of (Côte d’Ivoire),01 PO Box 490 Abidjan 01.

²Biochemistry-Microbiology Pedagogy Unit, University of Jean Lorougnon Guédé of Daloa, PO Box 150 Daloa

³Laboratory of Nutrition and Pharmacology, Faculty of Biosciences, University of Felix

Houphouet Boigny,Côte d’Ivoire. 22 PO Box 582 Abidjan 22 (Côte d’Ivoire).

⁴Laboratory of Biochemistry and Pharmacodynamy Faculty of Biosciences, University of Felix HouphouetBoigny, Côte d’Ivoire.22 PO Box 582 Abidjan 22 (Côte d’Ivoire).

⁵Institut Pasteur de Paris, Department of Parasites and Insect Vectors

⁶Laboratory of Fundamental Biochemistry, Institut Pasteur of Côte d’Ivoire, 01 PO Box 490 Abidjan 01.

*Corresponding author E-mail: [email protected]

Accepted 27 September, 2017

Abstract

Key words: Gametocytes, Plasmodium falciparum, hematocrit, isolates, parasitaemia, antiplasmodial.

African Journal of Parasitology Research ISSN 2343-6549 Vol. 4 (8), pp. 244-248, October, 2017. © International Scholars Journals

Full Length Research Paper

Eimeria species oocyst morphometry and prevalence of Infection in domesticated pigeon (Columba livia domestica) in Maiduguri Metropolis Borno State, Nigeria

Jonathan Joseph^{1, 2}, Wama Binga Emmanuel ², Vera Akwa Yafeh ², Ifeanyi Oscar Ndimkaoha Aguzie³*

¹Department of Biology, University of Maiduguri, Borno State, Nigeria.

²Department of Biology, Taraba State University, Taraba State, Nigeria.

³Department of Zoology and Environmental Biology, P.O. Box 3146, University of Nigeria, Nsukka, Enugu State, Nigeria.

Corresponding author's E-mail: [email protected]

Accepted 03 October, 2017

Abstract

Prevalence of Emeria species among domesticated pigeons (Columba livia domestica) in Maiduguri Metropolis was investigated. Floatation method was used to concentrate the coccidian oocysts using saturated sucrose solution. Sporulation technique was used to determine the size and morphological characteristics of the oocysts. Overall, 41 (40.6%) out of a total of 101 faecal samples examined were infected. Four parasite species under the genus Eimeria were isolated namely: E. columbae, E. tropicalis, E. columbarum, and E. labbeana; and at prevalence of 36.6%, 6.9%, 5.9% and 5.9% respectively. The difference in prevalence of the parasite species was significant (χ² = 76.577, p < 0.0001). Young pigeons were significantly more infected (53.1%) than adults (28.2%) (p < 0.05). E. tropicalis oocysts had the largest size (21.5µm by 20.5µm) followed by E. labbeana oocyst (16.5µm by 15.0µm). E. columbae had the longest sporulation time of 96 hours while E. labbeana had the shortest, 24 hours. This study provides information on the prevalence and veterinary significance of Eimeria oocysts of pigeons in Maiduguri Metropolis.

Keywords: Coccidian, Eimaria labbeana, Eimeria oocyst morphometry, sporulation time.

African Journal of Parasitology Research  ISSN 2343-6549 Vol. 4 (7), pp. 235-243, September, 2017. © International Scholars Journals

Full Length Research Paper

Associations between IgG antibody responses to multiple Plasmodium falciparum antigens and treatment outcomes with ACTs in Man, Cote d’Ivoire

Yao SS¹, Offianan AT¹, Tiacoh NL¹, Ako AAB¹, Koffi D¹, Kouame E¹, Tuo K¹, Beourou S¹, Djaman J²

¹Malariology Department, Institut Pasteur Côte d’Ivoire.

²Biochemestry Department Institut Pasteur/UFR Biosciences Félix Houphouët Boigny University.

Corresponding Author E-mail: [email protected]

Tel. (00225) 40 33 57 83/ (00225) 49 45 91 03

Accepted 18 September, 2017

Abstract

Successful antimalarial drugs treatment might be due to intrinsic susceptibility of P. falciparum and host’s factors. In this study we assessed relationship between host IgG antibody responses and treatment outcome with Artesunate+Amodiaquine (AS+AQ) and Artemether+Lumefantrine (AL) in high transmission setting of Cote d’Ivoire. The study was done as part of a clinical drug-efficacy trial of AS+AQ and AL conducted at Man in western Côte d’Ivoire. Serum samples from the time of malaria diagnosis and 7 days later were analyzed for total IgG against schizonte extract (07/03) and 5 specific-P. falciparum Ags using a quantitative indirect ELISA. IgG antibody responses were related to treatment outcome. A total of 56 and 55 serum samples in AL and AS + AQ group respectively were available for antibody analysis, on day 0 and day 7.  The levels IgG to CSP, GLURP and SALSA increased significantly with age (p<0.05). Antibody responses against all antigens decreased significantly, except SALSA and sch07/03 in day 7 after treatment, with the greatest decreasing seen in IgG response to GLURP (p= 0.0013). Adequate Clinical and Parasitological Response PCR corrected (ACPR) was of 98.3% (AS +AQ) and 100% (AL). Prevalence and levels of anti-Glutamate-rich Protein (GLURP) and anti-circumsporozoite (CSP)-specific IgG antibodies were significantly higher (P < 0.001) in Patients with ACPR in AS+AQ and AL group respectively.  GLURP and CSP IgG antibody responses in Man region may contribute to malaria treatment success and support hypothesis that acquired immunity enhances clinical efficacy of antimalarial dugs.

Keywords: IgG Antibody responses, treatment outcome, ACT, Man, Côte d’ivoire

African Journal of Parasitology Research  ISSN 2343-6549 Vol. 4 (6), pp. 227-233, June, 2017. © International Scholars Journals

Full Length Research Paper

Assessment of diagnostic performances of parasitological stool examinations performed in public sector medical laboratories of Abidjan (Côte d’Ivoire)

Konaté A^1*, Kiki-Barro PCM¹, Kassi KF^1,3, Djohan V¹, Angora KE¹, Bosson-Vanga H¹, Doukouré CY¹, Yavo W¹ and Menan EIH^1,3

¹Department of Parasitology, Mycology and Zoology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d'Ivoire.

²Laboratory of Parasitology and Mycology, Yopougon Teaching Hospital, 21 BP 632  Abidjan 21, Côte d'Ivoire.

³Laboratory of Parasitology and Mycology, Center for Diagnosis and Research on AIDS and  other infectious diseases, 01 BPV 13, Abidjan, Côte d'Ivoire.

E-mail: [email protected] Tel: +22507571931;+22540239134

Accepted 08 June, 2017

Abstract

Parasitological stool examinations allows to identify intestinal parasites in order to achieve a better management. Their importance implies the need to ensure high reliability and to seek perfection in their performance. We therefore carried out this study in order to update data on the diagnostic performance of public sector medical laboratories of Abidjan with regard to the research and identification of helminths and protozoa contained in stools. Our study was led from April to November 2011. Each participating laboratory received a survey sheet for the collection of information and two suspensions of stools to be analyzed. Our results showed a participation rate of 78.4 %. The responsibility for laboratories was entrusted to technicians and engineers in 62.1 % of cases. The written operating procedures were absent in 34.5 % of cases. We obtained for a correct identification estimated at 10.3 % and 89.7 % respectively for samples No.1 and No.2. Strongyloides Stercoralis and Entamoeba coli were the most recognized (69%). Confusion was noted between the various species of parasites in both suspensions. This study shows the importance of the application of existing texts on quality, in particular on the external quality assessment which must be compulsory at the national level.

Keywords: Quality control, intestinal parasites, medical laboratory.