African Journal of Nursing and Midwifery

African Journal of Nursing and Midwifery ISSN 2198-4638 Vol. 7 (7), pp. 001-002, July, 2019. © International Scholars Journals

Editorial

The digitization of medical records

Key words: Tubulin, expression, regulation.

African Journal of Nursing and Midwifery ISSN 2198-4638 Vol. 7 (6), pp. 001-002, June, 2019. © International Scholars Journals

Editorial

Health disparity– a concern

African Journal of Nursing and Midwifery ISSN 2198-4638 Vol. 7 (6), pp. 001-007, June, 2019. © International Scholars Journals

Full Length Research Paper

Effects of soy protein on selected enzymes in tissues of rats fed a cholesterol diet

Olarewaju M. Oluba^1*, E. Chukwu Onyeneke¹, Godwin C. Ojieh² and George O. Eidangbe²

¹Department of Biochemistry, University of Benin, P. M. B. 1154, Benin-City, Nigeria.

²Department of Medical Biochemistry, College of Medicine, Ambrose Alli University, Ekpoma, Nigeria.

Accepted 24 March, 2019

Abstract

Soy protein, an important component of soybeans is unique among plant-based protein because of its associated isoflavones. Isoflavones are a group of compounds with variety of biological properties that may potentially benefit human health. Their actions in various tissues have motivated researchers to access the possible related mechanisms and functions. This study is aimed at determining the effect of soy protein on selected tissue enzymes (used as aids in early diagnosis of cardiovascular disease) in rats fed a cholesterol diet. 24 male Wistar albino rats were assigned randomly into three groups. The first group serving as control was placed on normal diet while groups two and three were fed diet containing 5% cholesterol and 5% cholesterol plus 5% soy protein, respectively. The animals were placed on their respective diet for 7 weeks and at weekly interval LDH, ALT, AST and - GT activities in the liver, kidney and heart were monitored. At the end of the study period, LDH, ALT, AST and -GT activities were substantially reduced in the liver, kidney and heart of rats fed soy protein plus cholesterol diet compared with those fed cholesterol diet without soy protein. These results indicate that soy protein reduces the accumulation of excess fat in the liver, kidney and heart and thus prevent cell death due to lipotoxicity.

Key words: Soy protein, cholesterol, diet, enzyme, lipotoxicity, cardiovascular disease.

African Journal of Nursing and Midwifery ISSN 2198-4638 Vol. 7 (6), pp. 001-002, June, 2019. © International Scholars Journals

Editorial 

Research and Development of Drugs – unequal for certain diseases

African Journal of Nursing and Midwifery ISSN 2198-4638 Vol. 7 (5), pp. 001-004, May, 2019. © International Scholars Journals

Full Length Research paper

Evaluation of the association of NOD2/CARD15 gene polymorphisms with clinical course of Turkish Crohn’s disease patients

Fatih Tekin¹*, Afig Berdeli², Omer Ozutemiz¹, Ahmet Aydin¹, Ahmet Musoglu¹, Necla Osmanoglu¹ and Tankut Ilter¹

¹Ege University Medical School, Department of Gastroenterology, Izmir, Turkey.

²Ege University Medical School, Department of Pediatrics, Division of Genetics, Izmir, Turkey.

Accepted 17 March, 2019

Abstract

NOD2/CARD15 gene variants may be associated with distinct phenotypic expressions of Crohn’s disease, however, this association may change according to the ethnic and regional variation. The aim of this study was to analyze the impact of NOD2/CARD15 gene mutations on disease phenotype in Turkish Crohn’s disease patients. Fourty-five Crohn’s disease patients (32 males, 13 females) with a mean age of 38.7 ± 12.1 (range: 19-78) were enrolled into this prospective study. The three major polymorphisms (R702W, G908R, 3020insC) on NOD2/CARD15 gene were studied from the peripheral blood genomic DNA. R702W and G908R mutations were studied by PCR-RFLP method, and 3020insC mutation was studied by DNA sequencing. No homozygous mutation was detected. Heterozygous R702W, G908R, and 3020insC mutations were detected in 4, 3, and 4 patients, respectively. The frequency of R702W, G908R, and 3020insC mutations was found to be 4.4, 3.3, and 4.4%, respectively. The overall mutation frequency was found to be 12.2%. There was no statistically difference between the clinical course of the patients with (n = 11) and without (n = 34) mutations (p>0.05). NOD2/CARD15 gene polymorphisms do not have impact on disease phenotype in Turkish Crohn’s disease patients.

Key words: NOD2/CARD15 gene, Crohn’s disease, phenotype.