ISSN 2756-3286
Advances in Food Science and Technology ISSN: 6732-4215 Vol. 12 (5), pp. 001-010, May, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Optimizing Benzimidazole Extraction with Supported Liquid Membranes: A Chemometric Study of Influential Factors
Titus A. M. Msagati1*, J. Catherine Ngila1, Mathew M. Nindi2 and Bhekie B. Mamba1
1Department of Chemical Technology, Faculty of Science, University of Johannesburg, PO Box 17011, Doornfontein,
2028, South Africa.
2Department of Chemistry, University of South Africa, P. O. Box 392, Pretoria, 0003 South Africa.
Accepted 19 November, 2023
Abstract
Principle component analysis (PCA) and a two-way analysis of variance (ANOVA) were employed in the study of factors affecting extractability of benzimidazole anthelmintics using supported liquid membrane (SLM) in liver, kidney, milk and urine at four concentration levels. The SLM extraction process was monitored by liquid chromatography - mass spectrometer (LC-MS). The results showed that the extractability of benzimidazoles is dependent on both the concentration levels and the chemical properties of compounds. Based on chemical properties, extraction of the compounds from the liver matrix showed no significant difference (p = 0.05) for the following pairs; albendazole and oxibendazole, thiabendazole and mebendazole, oxibendazole and fenbendazole, and oxibendazole and mebendazole. At some of the concentration levels, mainly between 1000 and 100, 100 and 10, and 10 and 1 µg/Kg, there was no significant difference. It was also found that, there was significant difference (at p = 0.05) in the extractability in milk between oxibendazole and albendazole, and also oxibendazole and fenbendazole. For milk also, the concentration range from 10 to 100 µg/L, showed no significant difference (p = 0.05). Urine matrix on the other hand, showed significant difference in the recoveries at all concentration levels.
Key words: Benzimidazoles, supported liquid membrane (SLM), liquid chromatography-mass spectrometry (LC-MS), two-way analysis of variance (ANOVA), principle component analysis (PCA).