Advances in Food Science and Technology

Advances in Food Science and Technology ISSN: 6732-4215 Vol. 5 (4), pp. 001-004, April, 2017. © International Scholars Journals

Full Length Research Paper

Proteolytic and lipolytic behavior of Pseudomonas spp. secluded from pasteurized milk

*Mohammed E. Omar, Rames Nefertiti and Hamama Dalida

Department of Microbiology and Environmental Biotechnology, Mansoura University, Mansoura, Egypt.

Accepted 18 January, 2017

Abstract

Psychrotrophic bacteria have been recognized as a recurring problem in refrigerated storage and distribution of perishable food products. This study was performed to isolate, characterize and evaluate the prevalence of Pseudomonas spp. in pasteurized milk. Thirty five (35) pasteurized milk samples were collected aseptically from different dairy shops, supermarkets and groceries in different areas in Sadat City, Menuofiya Governorate, Egypt and analyzed for total viable count and Pseudomonas spp. occurrence and explain their proteolytic and lipolytic activities. The results showed that Pseudomonas aeruginosa was found to be the predominant from the Pseudomonas spp. followed by Pseudomonas fluorescens. The total viable count was (3.50 ± 0.085) × 10 ⁴ cfu/ml. Out of 10 Pseudomonas spp. isolates that were examined for proteolytic and lipolytic activities; two isolates had proteolytic activity, while three had lipolytic activity and five isolates had both.

Key words: Pseudomonas aeruginosa, Pseudomonas fluorescens, proteolytic, lipolytic activities.