ISSN 2756-3839
Research Article
International Journal of Nematology and Entomology Vol. 1 (6), pp. 117-120, November, 2013. © International Scholars Journals
Full Length Research Paper
Pseudomonas fluorescens as an efficient entomopathogen against Oligonychus coffeae Nietner (Acari: Tetranychidae) infesting tea
Amsalingam Roobakkumar1*, Azariah Babu1, Duraikkannu Vasantha Kumar1, Vattakandy Jasin Rahman1 and Soumik Sarkar2
1Division of Entomology, UPASI Tea Research Foundation, Tea Research Institute, Nirar Dam BPO,
Valparai 642 127, India.
2Division of Plant Pathology, UPASI Tea Research Foundation, Tea Research Institute, Nirar Dam BPO, Valparai 642 127, India.
*Corresponding author. E-mail: [email protected]. Tel: +91 4253-235301, 235303. Fax: +91 4253-235302.
Accepted 28 July, 2013
Abstract
The biocontrol efficacy of the bacterium, Pseudomonas fluorescens was evaluated against the red spider mite (RSM), Oligonychus coffeae in the laboratory. Both bacterial suspension and extra cellular filtrate of 24, 48 and 72 h old were sprayed to test the efficacy of bacterium against adult mites. The mortality due to P. fluorescens increased as the age and percentage of culture increased. Chitinase activity of P. fluorescens in response to chitin induction was measured in nutrient agar by estimating the amount of reducing sugars liberated. Chitinase activity was higher in 72 h old culture.
Key words: Pseudomonas fluorescens, red spider mite (RSM), tea, biocontrol, chitinase.
Amsalingam Roobakkumar, Vattakandy Jasin Rahman and Soumik Sarkar, Azariah Babu, Duraikkannu Vasantha Kumar
Research Article
Managing nematode pests and improving yield of pineapple with Mucuna pruriens in Ghana
International Journal of Nematology and Entomology Vol. 1 (4), pp. 074-077, September, 2013.© International Scholars Journals
Full Length Research Paper
Managing nematode pests and improving yield of pineapple with Mucuna pruriens in Ghana
K. Osei 1*, R. Moss2, A. Nafeo2, R. Addico, A. Agyemang1 and J.S. Asante1
1CSIR- Crops Research Institute, P. O. Box 3785, Kumasi, Ghana.
2West Africa Fair Fruit, P. M. B. KD11, Kanda, Accra, Ghana.
*Corresponding author. E-mail: [email protected]. Fax: +233-51-60396.
Accepted 3 August, 2013
Abstract
We investigated the potentials of a non-traditional legume crop, Mucuna pruriens and fallow treatments in managing plant parasitic nematodes population and improving the yield of pineapple in 2008 to 2009 at Asamankese in the Eastern region of Ghana. Nematode population density/200 cm3 soil at 6 months of application of treatments and at harvest, population/g-1 of pineapple root and yield of pineapple were analysed. M. pruriens reduced nematodes population and improved the yield of pineapple considerably. At harvest, rhizosphere soil from pineapple cultivated on bush fallow treated plots recorded significantly P = 0.05) higher populations: 76, 211, 100 and 68 for Helicotylenchus multicintus, Meloidogyne (juveniles) spp., Pratylenchus brachyurus and Rotylenchulus reniformis, respectively while soil from pineapple on weed free-M. pruriens treated plots recorded lower populations: 39, 50, 64 and 31 representing population reduction of 95, 322, 56 and 119%, respectively. Three nematode species: Meloidogyne spp., P. brachyurus and H. multicintus were recovered from pineapple roots at harvest in which the nematicidal potential of M. pruriens was further demonstrated. While significantly (P < 0.01) higher populations (165, 89 and 61) of Meloidogyne spp., P. brachyurus and H. multicintus were recovered from the bush fallow treated pineapple roots, lower populations (39, 35 and 24) of the pests were recovered from weed free-M. pruriens treated pineapple roots, respectively. Thus, M. pruriens reduced populations by 323, 154 and 154%, respectively. The yield improvement potential of M. pruriens was manifested when the main pineapple crop from weed free-Mucuna plots recorded 51 t/ha, whilst a bush fallow treatment recorded 34 t/ha out-yielding the bush fallow treatment by 50%. The use of higher plants in managing nematodes and improving yield of crops is environmentally acceptable and sustainable.
Key words: Ananas comosus, horticultural crop, plant parasitic nematodes, sustainable agriculture.
R. Addico, A. Nafeo, K. Osei, A. Agyemang and J.S. Asante, R. Moss
Research Article
Damage assessment and management of cucurbit fruit flies in spring-summer squash
International Journal of Nematology and Entomology Vol. 1 (4), pp. 064-068, September, 2013. © International Scholars Journals
Full Length Research Paper
Damage assessment and management of cucurbit fruit flies in spring-summer squash
R. Sapkota*, K. C. Dahal and R. B. Thapa
Institute of Agriculture and Animal Science Chitwan, Nepal.
*Corresponding author.E-mail: [email protected]
Accepted 13 August, 2013
Abstract
Cucurbit fruit fly, Bactrocera cucurbitae (Coquillett), is one of the most important pests of cucurbits, and squash (Cucurbita pepo Lin.) is highly prone to damage by this pest in Nepal. Because of the difficulties associated with the control of this pest by chemical insecticides, farmers experienced great losses in cucurbits. Therefore, a participatory field experiment was conducted under farmer field conditions to assess losses and to measure the efficacy of different local and recommended management options to address the problem of it in squash var. Bulam House (F1). The experiment consisted of six different treatments including untreated control, and there were four replications. All the treatments were applied 40 days after transplanting. Cucurbit fruit fly preferred young and immature fruits and resulted in a loss of 9.7% female flowers. Out of total fruits set, more than one-fourth (26%) fruits were dropped or damaged just after set and 14.04% fruits were damaged during harvesting stage, giving only 38.8% fruits of marketable quality. Application of locally made botanical pesticide ‘Jholmal’ was found superior in terms of fruit size (895 g), quality and yield (62.8 t/ha), and reduced fruit fly infestation in squash as compared to other treatments. Although, ‘Jholmal’ preparation is easy and its application is effective for the management of cucurbit fruit fly, it involves more labor cost and frequent application is a tedious process. Future efforts should be made to find the ways to reduce the cost of its application to make vegetable cultivation more profitable.
Key words: Bactrocera cucurbitae, Cucurbita pepo, pesticide, melon fly, food baits.
R. Sapkota, K. C. Dahal and R. B. Thapa
Research Article
International Journal of Nematology and Entomology Vol. 1 (3), pp. 042-052, August, 2013. © International Scholars Journals
Full Length Research Paper
Annotated catalogue of whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) from Arasbaran, Northwestern Iran
Hassan Ghahari1*, Shaaban Abd-Rabou2, Jiri Zahradnik3 and Hadi Ostovan4
1Department of Agriculture, Islamic Azad University, Shahre Rey Branch, Tehran, Iran.
2Plant Protection Research Institute, Ministry of Agriculture, Dokki-Giza, Egypt.
3Podebradova 498, 512 51, Lomnice nad Popelkou, Czech Republic.
4Department of Entomology, Islamic Azad University, Fars Science and Research Branch, Iran.
*Corresponding author. E-mail: [email protected].
Accepted May 1, 2013
Abstract
The fauna of whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) was studied in Arasbaran region, Northwestern Iran. A total of 25 species of 15 genera were collected which of these, two species including, Tetralicia ericae Harrison and Trialeurodes ericae Bink-Moenen are new records for Iran.
Key words: Aleyrodidae, fauna, new record, arasbaran, Iran.
Shaaban Abd-Rabou, Hassan Ghahari, Jiri Zahradnik and Hadi Ostovan
Research Article
Phytosanitary irradiation in South Asia
International Journal of Nematology and Entomology Vol. 1 (5), pp. 093-101, October, 2013. © International Scholars Journals
Full Length Research Paper
Phytosanitary irradiation in South Asia
M. Aftab Hossain1, J. Guy Hallman2*, A. Shakil Khan1 and M. Saidul Islam1
1Insect Biotechnology Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar,
Dhaka 1349, Bangladesh.
2USDA-ARS, 2413 E. Highway 83, Weslaco, TX 78596, USA.
*Corresponding author. E-mail : g[email protected]. Tel: 956 447-6313. Fax: 956 447-6323.
Accepted 23 October, 2013
Abstract
Irradiation has the potential to solve phytosanitary problems related to trade in South Asia. In general, it is the phytosanitary treatment most tolerated by fresh agricultural commodities. Irradiation technology is available in some countries of the region but is only used for phytosanitary purposes for mangoes in India exported to the United States of America since 2007. In this review, we analyze the literature on phytosanitary irradiation, present the protocol for exporting irradiated Indian mangoes, suggest possibilities for the technology in South Asia and discuss further research needs.
Key words: Radiation disinfestation, quarantine treatment, commodity quality, mango export.
M. Aftab Hossain, J. Guy Hallman, A. Shakil Khan and M. Saidul Islam
Research Article
International Journal of Nematology and Entomology Vol. 1 (5), pp. 089-092, October, 2013.© International Scholars Journals
Full Length Research Paper
Nematicidal effect of Acacia nilotica and Gymnema sylvestris against second stage juveniles of Meloidogyne incognita
Nighat Sultana1*, Musarrat Akhter2, Muhammad Saleem3 and Yousaf Ali1
1Pharmaceutical Research Center, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories
Complex, Karachi-75280, Pakistan.
2Food and Marine Resources Research Centre, Pakistan Council of Scientific and Industrial Research (PCSIR)
Laboratories Complex, Karachi-75280, Pakistan.
3Department of Chemistry, the Islamia University of Bahawalpur, Pakistan.
*Corresponding author. E-mail: [email protected]. Tel: (92-21) 8141832-35. Fax: (92-21) 8141847.
Accepted 23 September, 2013
Abstract
The nematicidal effect of different extracts of Acacia nilotica leaves/seeds and Gymnema sylvestre leaves were tested against Meloidogyne incognita larvae at the concentrations of 1, 0.5, 0.25 and 0.125%, up to three days. All extracts showed nematicidal mortality. Nematicidal mortality was 100% with the use of 1% concentration of leaves ethyl alcohol extract of G. sylvestre and ethyl acetate leaves extracts of A. nilotica after 2 days. Qualitative analysis of the phytochemicals of alcohol extracts revealed the presence of carbohydrates, saponins, triterpene saponins belonging to oleanane and dammarene, phytosterols, phenols, flavonoids and tannins in all the plants. Quantitative analysis showed that, the crude saponin was the major phytochemical constituent present in highest percentage followed by crude oleanane and dammarene triterpene acids in both of two plants. It is suggested that both of these two plants possess nematicidal properties that could be developed and used as natural nematicides for nematode control.
Key words: Acacia nilotica, nematicidal activity, ellagic acid, oleanolic acid 3-glycosides, quercetin 3-glycosides, 3,19-dihydroxy-12-ursen-28-oic acid.
Musarrat Akhter, Nighat Sultana, Muhammad Saleem and Yousaf Ali