African Journal of Crop Science

African Journal of Crop Science ISSN 2375-1231 Vol. 5 (7), pp. 001-006, July, 2017. © International Scholars Journals

Full Length Research Paper

Linkage mapping of lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers

Bahattin Tanyolac^1*, Sehnaz Ozatay¹, Abdullah Kahraman² and Fred Muehlbauer³

¹Ege University, Department of Bioengineering, 35100 Bornova-Izmir Turkey.

²Harran University, Department of Field Crops, S. Urfa, Turkey.

³Department of Crop and Soil Sciences, USDA-ARS, Washington State University, 303 Johnson Hall, Pullman, WA99164-6434, USA.

Accepted 22 January, 2017

Abstract

We have constructed a molecular linkage map of Lentil (Lens culinaris L.) in a RIL population (developed from the parents Precoz x WA8949041) of 94 plants with 166 markers. The resulting linkage map consists of 11 (varying between 50.9 – 436.5 cM) linkage groups covering 1396.3 cM with an average map distance between framework markers of 8.4 cM. Most of the RAPD and ISSR markers were evenly distributed across the genome. By using 40 primer combinations, we were able to generate 391 AFLP markers in the RIL population. The majority of the AFLP markers clustered in linkage group 1. In the segregation distortion analysis, 185 markers deviated from Mendelian segregation. Of the 185 markers with skewed segregation, 108 markers were unlinked and the rest of the distorted markers mapped to linkage groups. LG1 contained the most distorted marker among the linkage groups.

Key words: AFLP, RAPD, ISSR, Lentil, Segregation Distortion, Linkage map.