ISSN 2756-3707
International Journal of Histology and Cytology Vol. 1 (10), pp. 091-092, October, 2014. © International Scholars Journals
Full Length Research Paper
Pterocarpus osun extract as a histological stain for collagen fibres
Abubakar Bukar*, Hammed Olla and Harshanti O.
Department of Medical Laboratory Science, Histopathology Unit, Faculty of Health Science and Technology University of Port Harcourt, P. M. B. 5323, Port Harcourt, Nigeria.
E-mail: [email protected]
Accepted 11 September, 2014
Abstract
The staining ability of Pterocarpus osun extract on tissue sections was determined. 2 kg of P. osun stem was dried, milled to obtain a fine powder and a red pigment extracted from the powder with 1 L of 70% ethanol at 78°C for 24 h. The alcoholic alkaline and acidic extracts were used to stain tissue sections. Collagen fibres, red blood cells and muscles were stained in shades of reddish brown. Preliminary phytochemical screening of this extract revealed that it contained alkaloids, steroid ring, cardiac glycosides and reducing sugar.
Key words: Pterocarpus osun, phytochemical screening, tissue sections, stain, mordant.