ISSN 2167-0447
International Journal of Agricultural Sciences ISSN 2167-0447 Vol. 10 (12), pp. 001-006, December, 2020. © International Scholars Journals
Full Length Research Paper
Identification of genes involved in rice seed germination at low temperatures
Demócrito Amorim Chiesa Freitas1, SilmarTeichert Peske2, Francisco Amaral Villela2, Paulo Dejalma Zimmer2, Paulo Ricardo Reis Fagundes3, Fernanda Plucani do Amaral4, Elaine Gonçalves Rech5 and Geri Eduardo Meneghello1*
1Post Graduation Seeds Science and Technology, FAEM-UFPel, Campus s/n, C. P. 354, Zip Code 96010-900 Pelotas, RS, Brazil.
2Post Graduation Seed Science and Technology, FAEM - UFPel – Campus, Pelotas, RS, Brazil.
3Researcher of Embrapa Clima Temperado. Pelotas, RS, Brazil.
4Post Graduation Seed Science and Technology, FAEM - UFPel, Pelotas, RS, Brazil.
5Post-Doctoral in Seed Science and Technology, FAEM - UFPel, Pelotas, RS, Brazil.
Accepted 22 November, 2020
Abstract
Rice is a model for many genomic studies among crop species. The adaptation to different crop environments is partly the result of traditional breeding combined with molecular assisted selection. Comparative studies between contrasting genotypes can be performed efficiently by the cDNA-AFLP technique, allowing the identification of genes involved in the expression of the target trait. The objective of this study was to identify differentially expressed genes in cultivars contrasting for the character germination at low temperatures. The genotypes used were BRS-Firmeza (Tolerant) and SCSBRS Tio-Taka (Sensitive). Seeds of the two cultivars were sown at 13 and 25°C, respectively. Total RNA was extracted from embryos and cDNAs were synthesized. The cDNAs were analyzed by AFLP (Amplified Fragments Length Polymorphisms) to identify differentially expressed sequences between BRS-Firmeza and SCSBRS Tio-Taka varieties. Four polymorphic fragments were present at 13°C IN BRS-Firmeza molecular profile and absent in SCSBRS Tio-Taka. It is concluded that the technique of AFLP on cDNA as template is efficient to produce and identify fragments of differentially expressed genes in rice during germination under low temperatures.
Key words: Oryza sativa, seeds, cDNA-AFLP (cDNA amplified fragment length polymorphism).