ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2756-3707 Vol. 9 (1), pp. 001-007, January, 2022. © International Scholars Journals
Full Length Research Paper
Anti-ulcerogenic activity of Gynura procumbens leaf extract against experimentally-induced gastric lesions in rats
A. A. Mahmood1*, Abdalbasit A. Mariod2, Fouad Al-Bayaty3 and Siddig Ibrahim Abdel-Wahab4
1Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
2Department of Food Science and Technology, College of Agricultural Studies, Sudan University of Science and Technology, P.O. Box 71, Khartoum North, Sudan.
3Department of Restorative Dentistry, Faculty of Dentistry, University Technology Mara, 40000, Shah Alam, Selangor, Malaysia.
Accepted 25 November, 2021
Abstract
Several plants are used in folk medicine to treat gastrointestinal disorders. Gynura procumbens is a medicinal plant commonly used in traditional treatment of many aliments. In this study, G. procumbens ethanolic leaf extract (GPELE) was used to investigate its gastroprotective effect in adult Sprague dawley rats which were divided into six groups. The rats were orally pre-treated with carboxymethyl cellulose (CMC) solution (ulcer control groups), omeprazole 20 mg/kg (reference group), 50, 100, 200 and 400 mg/kg of GPELE in CMC solution (experimental groups), one hour before oral administration of absolute ethanol to generate gastric mucosal injury. After an additional hour, the rats were sacrificed and the ulcer areas of the gastric walls were determined. The ulcer control group exhibited severe mucosal injury, whereas groups pre-treated with GPELE exhibited significant protection of gastric mucosal injury. These findings were also confirmed by histological studies. Acute toxicity study with a higher dose of 5 g/kg did not manifest any toxicological signs in rats. These results suggest that GPELE promotes ulcer protection as ascertained grossly by significant reduction of ulcer area, and histologically by comparatively decreases in ulcer areas, reduction or absence of edema and leucocytes infiltration of submucosal layer compared to ulcer control group.
Key words: Gynura procumbens, cytoprotection, gastric ulcer, histology, ethanolic extract.