African Journal of Food Science Research

ISSN 2375-0723

African Journal of Food Science Research ISSN 2375-0723 Vol. 6 (5), pp. 314-320, May, 2018. © International Scholars Journals

Full Length Research Paper 

A study of the physiochemical and microbiological analysis of the commercial milk market

Ammara Hassan1*, Imran Amjad2 and Shahid Mahmood1

1Applied Chemistry Research Centre, Pakistan Council of Scientific and Industrial Research, Lahore, Pakistan

2Reaearch and Development Department, Haleebfoods (Pvt) Ltd, Lahore, Pakistan

*Corresponding author. E-mail: [email protected].

Accepted 05 February, 2018

Abstract

The aim of the study was to diagnose physiochemical, microbiological, sensorial changes and to differentiate the milk collection systems of the commercial milk market competitor during storage of 12 weeks. The parameters used for physicochemical analysis are; sedimentation, solids non fats (SNF), fat and protein %, total titratable acidity and pH and for microbiological analysis; total plate, coliform, Bacillus cereus and Bacillus subtilus, Escherichia coli and spore forming bacterial count were determined. Colour, taste and aroma were observed during storage. The results strongly reflect an increase in sedimentation value with the ice mixing or dilution before processing which disturbed the salt balance, protein charges and natural emulsion. There was increase in acidity and sedimentation of milk but pH, % of fat contents, SNF (solids non fats) and proteins decreased during storage. The negative changes occurred in colour, aroma and flavor with reference to these physiochemical changes. Microbial counts for coliforms (e.g. E. coli), B. cereus, B. subtilis and heat resistant spores forming bacteria were zero. These all factors collectively limited the shelf life of UHT (ultra-high temperature) milk.

Key words: Physiochemical, microbiological, sensorial changes, heat resistant spores forming bacteria, UHT milk.