ISSN 2756-3278
Advances in Aquaculture and Fisheries Management ISSN 2756-3278 Vol. 12 (6), pp. 001-006, June, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Optimizing Storage Conditions for Kutum (RUTILUS FRISII KUTUM) Ova: Effects of Duration and Temperature
Azin Mohagheghi Samarin1*, Bagher Mojazi Amiri2, Mehdi Soltani3, Rajab Mohammad Nazari4, Abolghasem Kamali1 and Mohammad Reza Naghavi5
1Department of Aquaculture and Fisheries Science, Faculty of Agriculture and Natural Resources, Science and
Research Branch, Islamic Azad University, Tehran, Iran.
2Department of Fisheries and Environmental Science, Faculty of Natural Resources, University of Tehran, 31585-4314,
Karaj, Iran.
3Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, 14155-6453, Tehran, Iran.
4Nasr Fish Farm, Sari, Iran.
5Department of Plant Breeding, Faculty of Agriculture, University of Tehran, 31587-77871, Karaj, Iran.
Accepted 24 June, 2024
Abtract
Effects of post-stripping oocyte ageing and temperature on egg viability rates were studied in kutum (RUTILUS FRISII KUTUM). Eggs were retained outside the ovarian cavity (IN VITRO storage) with ovarian fluid at various temperatures of 4, 10, 12 and 26°C. Stored ova of six female kutum were fertilized separately after 0 (control eggs fertilized prior to storage), 2, 4, 6, and 8 h post-stripping (HPS) using fresh and pooled sperm obtained from five males. Eyeing, hatching and eyed egg mortality rates were recorded as an index of egg viability. The results indicated that the maximum eyeing (87%) and hatching (75%) rates of eggs took place at 0 HPS followed by 8 HPS (> 80 and > 70%, respectively) at 4°C. As storage temperature increased, egg viability decreased to 80, 70 and 50% viable at 8 HPS at 4, 10 and 12°C, respectively. The eggs stored at 26°C lost their viability almost completely after 4 HPS. Eyed-egg mortality increased from 13% at 0 HPS to 48.2% and at 4 HPS, at 26°C. This study demonstrated that the IN VITRO storage method is an applicable effective fertilization technique that can be used for restocking programs of kutum within 8 h at temperatures ranging from 4 to 12°C.
Key words: Rutilus frisii kutum, egg storage, temperature, ovarian fluid.