ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2006
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2006 International Scholars Journals
Full Length Research Paper
Activity enhancement of ligninolytic enzymes of Trametes versicolor with bagasse powder
Sk Masud Hossain1* and N. Anantharaman2
1Department of Chemical Engineering, Mohamed Sathak Engineering College, Kilakarai 623 806, India.
2Department of Chemical Engineering, National Institute of Technology, Tiruchirapalli 620 015, India.
Accepted 4 November, 2005
Abstract
Suspended cultures of white-rot fungus, Trametes versicolor, supplemented with bagasse powder showed a concentration dependent enhancement in the ligninolytic enzymes activity in liquid shake cultures. 2% (w/v) bagasse powder improved greater stability to the enzymes. The optimum pH is 3.5 and the optimum temperature is 40°C for maximum lignonolytic enzymatic activity. The optimum shaking speed is 60 rpm for maximum enzymatic activity. The maximum enzymatic activity showed by T. versicolor is 495, 440 and 410 mmol/ml.min for LiP, MnP and laccase with bagasse powder at optimum conditions, respectively. Without bagasse powder at optimum conditions, the maximum enzymatic activity for LiP, MnP and laccase is 195, 150 and 170 mmol/ml.min, respectively.
Key words: Bagasse, enhancement, enzymes, optimum, white-rot fungus.