ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN: 2169-3048 Vol. 5 (5), pp. 718-724, May, 2016. © International Scholars Journals
Full Length Research Paper
Varieties of ligno-cellulolytic enzymes and proteins of wood ear mushroom
Indira R. Modi1*, Ranvid C. E1, Rupeert Cindura2 and Vijay Shan1
1Department of Botany, University of Kashmir, India.
2Department of Botany, Bundelkhand University, Jhansi, U.P. India.
E-mail: [email protected]
Accepted 22 April, 2016
Abstract
The enzyme potential of different mushrooms varied differently and differed greatly based on the nature of the substrates used for cultivation. But in common, all mushrooms produce both cellulases and laccase, more particularly, for easy degradation and utilization of the substrate. High level of cellulolytic enzyme activities namely: exo β-1, 4 glucanase and endo β-1,4 glucanase was observed in beds prepared with paddy straw + wheat bran (3:1), whereas high level of lignolytic enzyme activity namely, laccase was observed in paddy straw + wheat bran (1:1). Protein profile studies of Auricularia polytricha were compared with those of Pleurotus platypus, Pleurotus florida, and Pleurotus eous. SDS - PAGE analysis of mycelium of different mushrooms revealed the presence of 12-14 protein bands in A. polytricha. Common protein bands were expressed at 14 kDa, 25 kDa and 49 kDa in all the mushrooms.
Key words: Auricularia polytricha, bioefficiency, cellulases, laccase, protein.