ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (8), pp. 001-006, August, 2018. © International Scholars Journals
Full Length Research Paper
Preliminary screening of antimycobacterial effect of psychrophillic Actinomycetes isolated from Manali ice point: Himachal predesh
A. Raja1* and P. Prabakaran2
1Department of Microbiology, Jamal Mohamed College, Tiruchirapalli, Tamilnadu, India.
2Department of Microbiology, PRIST University, Tanjavur, Tamilnadu, India.
Accepted 10 June, 2018
Abstract
Totally, 128 psychrophilic actinomycetes were isolated and 14 were found to be facultative psychrophilic which were selected to determine the antimycobacterial activity against Mycobacterium tuberculosis. The results showed that, four strains were active against the test organism M. tuberculosis. The active isolates screened in the present study were found to be highly effective and comes under Streptomycetes species (RH7 and RH8), Micromonospora species (RH9) and Micropolyspora species (RH12). All the isolates capable of producing metabolites and their presence were confirmed by TLC. The ‘active culture filtrate’ showed ‘only one band’ and its functional Group Is low molecular weight neutral compounds and amines were determined based on their solubility and pH.
Key words: Psychrophilic actinomycetes, glaciers, drug resistance, Mycobacterium tuberculosis, primary amines.