ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (5), pp. 001-005, May, 2019. © International Scholars Journals
Full Length Research Paper
A simplified system without purification for selection of aptamers against Vibrio alginolyticus
Jumin Hao, Jiang Zheng*, Xuemin Tang, Qingpi Yan, Yubao Li and Zhongbao Li
Fisheries College of Jimei University, Key Laboratory of Science and Technology for Aquaculture and Food Safety in Fujian Province University, Xiamen 361021, China.
Accepted 22 March, 2019
Abstract
We have explored a simplified system without purification for systematic evolution of ligands by exponential enrichment (SELEX) against Vibrio alginolyticus to select single-stranded DNA ligands (aptamers) from a random 82-nt library. The DNA content was quantified by agarose gel electrophoresis and follow-up image analyses with the BandScan 5.0 software. Anti-digoxigenin/HRP system was used to determine the binding activity of aptamers for V. alginolyticus. The results showed that the affinity of aptamers increased gradually with the increase of screening rounds, indicating that the lack of purification did not affect screening results but rather made SELEX screening much more convenient and significantly increased the efficiency.
Key words: Aptamers, purification, systematic evolution of ligands by exponential enrichment (SELEX), Vibrio alginolyticus.