ISSN 2736-1624
Frontiers of Agriculture and Food Technology ISSN: 2736-1624 Vol. 11 (2), pp. 001-006, February, 2021. © International Scholars Journals
Full Length Research Paper
Evaluation of anthelmintic activity of the stem bark extract and chemical constituents of Bridelia ferruginae (Benth) Euphorbiaceae
Lasisi A. A.1* and Kareem S. O.2
1Drug Research Unit, Department of Chemistry, University of Agriculture, Abeokuta, Ogun State, Nigeria.
2Department of Microbiology, University of Agriculture, Abeokuta, Ogun State, Nigeria.
Accepted 15 June, 2020
Abstract
Anthelmintic properties of the stem bark extract and compounds isolated from Bridelia ferruginae were investigated. In relation to the traditional use of B. ferruginae against gastro-intestinal infections, bioactivity-guided fractionations of the CHCl3- and CH 2Cl2- soluble fractions of the 80% MeOH extract from the stem barks of B. ferruginae yielded two known triterpenoids: betuline (1), glucoside of betulinic acid (2) and other two known flavonoids: quercetin (3) and kaempferol (4). Structures of compounds 1 to 4 were elucidated by spectroscopic studies and comparison with related compounds in literature. The time of paralysis and death of the parasitic worms: Fasciola gigantical (liver fluke), Taenia solium (tape worm) and Pheritima posthuma (earthworm, Annelid) were determined at 25, 50, 80 and 100 mg/ml. The stem barks extract of B. ferruginae and isolated compounds demonstrated concentration- dependent anthelmintic potencies against parasitic worms assayed. Structural-activity relationship is explained.
Key words: Bridelia ferruginae, anthelmintic activity, betulinic acid, betuline, quercetin, kaempferol.