ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced hematotoxicity in rabbits
A. Ologundudu1*, A. O.Ologundudu1, I. A. Ololade2 and F. O. Obi3
1Department of Biochemistry, Adekunle Ajasin University, P.M.B. 001, Akungba Akoko, Ondo State, Nigeria.
2Department of Chemistry and Industrial Chemistry, Adekunle Ajasin University, P.M.B.001, Akungba Akoko, Ondo State, Nigeria.
3Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Edo State, Nigeria.
Accepted 26 March, 2017
Abstract
In this study, the 2, 4-dinitrophenylhydrazine-induced biochemical and hematological changes in rabbits were examined under the administrative protocol of anthocyanin extract from Hibiscus sabdariffa calyces. Blood levels of reduced glutathione (GSH) and malondialdehyde (MDA) as well as red blood cell counts (RBC), white blood cell counts (WBC), packed cell volume (PCV) and hemoglobin (Hb) concentration were determined as indices of alteration and protection. Relative to control, 2, 4-dinitrophenylhydrazine (2, 4-DNPH) treatment significantly decreased (P < 0.05) blood level of GSH and significantly increased blood MDA level .It also significantly (P < 0.05) decreased RBC counts, PCV and Hb but increased WBC counts. On the other hand treatment of rabbits with Hibiscus anthocyanin extract led to significant (P < 0.05) increase in blood GSH, RBC counts, PCV and Hb and a decrease in MDA and WBC counts. These findings indicate that anthocyanin extract from dried calyces of H. sabdariffa protects the blood against 2, 4-DNPH lipoperoxidative and hemolytic effects.
Key words: Rabbit, Hibiscus sabdariffa , anthocyanin extract, 2,4 dinitrophenylhydrazine, reduced glutathione, malondialdehyde, complete blood count.