ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 5 (6), pp. 434-438, June, 2018. © International Scholars Journals
Full Length Research Paper
Effect of citric acid on the induction of cellulase production by the fungus under different cultural conditions
Prashanth Shanmugam*, Madhumathi Mani and Mathivanan Narayanasamy
Center for Advanced Studies in Botany, University of Madras, Chennai, 600025, India.
*Correspondingauthor.E-mail: [email protected].
Accepted 25 March, 2017
Abstract
TRICOTHECIUM ROSEUM, an unexplored fungus for the production of cellulolytic enzymes, was used in this study for carboxymethylcellulase (CMcase) and β-glucosidase production. The culture filtrate of this organism exhibited highest total cellulolytic activity and extracellular protein content on 9th day during the course of its growth on potato dextrose yeast extract (PDYE) broth medium supplemented with 1.0% (w/v) carboxymethylcellulose. Among various soluble carbon and complex nitrogen sources tested in this study, caboxymethylcellulose and peptone supported maximum production of both cellulolytic enzymes. Under all suitable growth conditions, the enzyme biosynthesis was remarkably increased when the inducer citric acid was added to the PDYE media containing carboxymethyl-cellulose. Maximum CMcase production (1.59 U/ml) and β-glucosidase production (1.82 U/ml) were achieved in citric acid amended PDYE media at 37°C. Both the cellulolytic enzyme production was highest at initial pH 6.5 and after 7 days incubation. The study clearly demonstrated that citric acid is a good inducer for extracellular cellulolytic enzyme production by the fungus.
Key words: Tricothecium roseum, cellulolytic enzymes, CMcase, β-glucosidase.