ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 7 (4), pp. 800-809, April, 2018. © International Scholars Journals
Full Length Research Paper
Optimization of microwave-assisted extraction of total phenol content and total flavonoids content from Anacardium occidental L. (Anacardeaceae) using response surface methodology
MADJITOLOUM BETOLOUM Salomon1,4*, TALLA Emmanuel2*, NGASSOUM Martin Benoît1, TSATSOP TSAGUE Roli Karole1, NYEMB Jean Noël2, MAHMOUT Yaya3
1Department of Applied Chemistry, National Advanced School of Agro-Industrial Sciences, University of Ngaoundere (UN), Cameroon.
2Department of Chemistry, Faculty of Science, University of Ngaoundere (UN), Cameroun.
3Laboratory of Research on Natural Substances, University of N’Djamena (UN), Chad.
4Department of Chemistry, Faculty of Sciences and Techniques, University Adam Barka of Abeche (UNABA), Chad.
Corresponding author: [email protected]; Tel.+23566305267; +237674863715;+237 699506524
Accepted 03 February, 2018
Abstract
The optimal conditions of Microwave-Assisted Extraction (MAE) of total phenol content and total flavonoids content from A. occidentale L. stem bark were determined. A second-order regression for central composite design (CCD) was used to investigate the effects of four independent variables, namely extraction time (s), irradiation power (W), solvent-to-solids ratio (mL/g) and methanol concentration (%) on the responses. The second-order regression for CCD consisted of 24 experimental points and 4 replications at the central point. Data were analyzed using Statgraphics software. The optimal conditions based on combination responses were: extraction time of 83 s, irradiation power of 620 W, solvent-to-solids ratio of 33.4 mL/g and methanol concentration of 63.17% according to the analysis of response surface. These optimum conditions yielded total phenolic contents (TPC) of 674.58 mg Gallic Acid Equivalent (GAE)/ 100g DM and total flavonoid content (TFC) of 85.38 mg Quercetin Equivalent (QE)/100g DM. Close agreement between experimental and predicted values was found.
Keywords: Anacardiu moccidentale L., total phenols and total flavonoids, Microwave-Assisted Extraction, Optimal conditions.