ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2006
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2006 International Scholars Journals
Full Length Research Paper
Factors affecting delivery and transient expression of -glucuronidase gene in Dendrobium Sonia protocorm-like-body
Janna OA1*, Maziah M2, Ahmad Parveez GKA4, Saleh K3
1Department of Microbiology, 2Department of Biochemistry, Faculty of Biotechnology & Biomolecular Sciences,
3Department of Horticulture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia.
4Malaysia Palm Oil Board, Biology Division, 50720 Kuala Lumpur, Malaysia.
Accepted 18 November, 2005
Abstract
The effect of the biolistic device parameters and other factors affecting delivery and expression of uidA gene in Dendrobium Sonia was investigated. Three week old protocorm like body (PLB) were bombarded with gold microparticles coated with pAHC25 plasmid harbouring the uidA gene which encodes -glucuronidase. The factors investigated were the helium pressure, target distance, macrocarrier flight distance to stopping screen, distance from stopping screen to target tissues, vacuum pressure, gold microparticles size, spermidine and calcium on DNA precipitation, and the number of bombardments. Two days after bombardment, the PLB were subjected to histochemical GUS assay, and transient GUS activity was recorded as blue spots using a Leica stereomicroscope. All the factors tested showed significant effects (p<0.05) on the delivery of DNA and expression of the uidA gene in Dendrobium PLB except for calcium. Surviving PLBs were able to grow and regenerate normally into plantlets.
Key Words: biolistic transformation, orchid, Dendrobium, protocorm like body,-glucuronidase assay.