ISSN 2375-1134
African Journal of Agriculture ISSN 2375-1134 Vol. 9 (6), pp. 001-003, June, 2022. Available online at https://internationalscholarsjournals.org/journal/ajaoas/table-of-contents © International Scholars Journals
Short Communication
Analysis of Ferulic Acid and Its Derivatives Using Thin Layer Chromatography
Leonard Vuyani Mabinya1*, Tungamirai Mafunga1 and John Morgan Brand2
1Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa.
2Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown 6140, South Africa.
Accepted 18 May, 2022
Abstract
The analysis of certain phenolic compounds from plants, and their chemical transformation with microorganisms or isolated enzymes, has application in the food and pharmaceutical industry. The rapid quantitative estimation of ferulic acid by thin layer chromatography is described by measurement of the area of the obtained spots. Furthermore, the qualitative analysis of a number of related phenolics, some of which are transformation products of ferulic acid, is achieved on thin layer chromatographic plates by the use of different spray reagents.
Key words: Phenolic compounds, quantitative, qualitative, ferulic acid, thin layer chromatography.