ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 18 (8), pp. 001-005, August, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Genetic Variability of Helicobacter pylori in Iran: Clarithromycin Resistance and 23S rRNA Mutations
Mohammad Kargar1*, Maryam Baghernejad1, Abbas Doosti2 and Sadegh Ghorbani-Dalini1,3
1Department 1Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
2Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
3Jahrom Branch, Young Researcher's Club, Islamic Azad University, Jahrom, Iran.
Accepted 12 April, 2024
Abstract
To determine the 23S rRNA point mutations in clarithromycin resistance of Helicobacter pylori strains isolated from southwest, Iran. This was a cross-sectional survey, which was done on 263 patients who referred to endoscopy department of Shehrekord university of medical sciences. According to gram stain, urease, catalase, oxidase and polymerase chain reaction (PCR) H. pylori identified. Standard National Committee for Clinical Laboratory Standard (NCCLS) method used for assessment of clarithromycin resistance. Specific primers and restriction enzymes BsaI and MboII by PCR-RFLP were used for analysis of A2143G and A2142G mutations. So for the detection of A2142C, specific primers and PCR method were used. 84 strains of H. pylori (31.94%) determined by PCR method. Of 19 (22.62%) clarithromycin resistant strains 13 (68.40%), 3 (15.78%), 2 (10.52%) had A2143G, A2142G, A2142C respectively and one unknown mutation in 23S rRNA gene. Because of considerable resistance to clarithromycin, direct diagnosis of this mutation by molecular approach in other parts of the country is necessary.
Key words: Polymerase chain reaction, clarithromycin, resistance, 23S rRNA, polymerase chain reaction - restriction fragment length polymorphism.