ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2756-3707 Vol. 7 (6), pp. 001-003, June, 2020. © International Scholars Journals
Short Communication
Effect of different plant hormones on callus induction in Gymnema sylvestris R.Br. (Asclepiadaceae)
Amitava Roy1, Shayak Ghosh1, Madhulina Chaudhuri1 and P. K. Saha2
1Plant Tissue Culture and Biotechnology Section, Department of Botany, Scottish Church College, 1 Urquhart Square, Kolkata-700006 India.
2Department of Botany, Bose Institute, 93/1 A.P.C. Road Kolkata 700009 India.
Accepted 03 June, 2020
Abstract
The use of Gymnema sylvestris as an alternative remedy for diabetes was well known from ancient times in India and the plant is valuable for its pharmaceutical properties. The present study describes callus induction of the plant G. sylvestris using internodal explants and the influence of different plant hormones like 2,4-D, kinetin, IAA, BAP on the growth of calli. Internodes were proved to be the best explant for culture, which were grown on MS basal medium (Murashige and Skoog, 1962) with different concentration of various growth regulators. The standard plant tissue culture protocol for callus culture and/or micropropagation was adopted. The highest efficiency of callus formation was observed in the medium containing different concentration of 2,4-D and kinetin.
Key words: Gymnema sylvestris, tissue culture, micropropagation, plant growth regulators, callus.