ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-008, February, 2019. © International Scholars Journals
Full Length Research Paper
Pulsed-field gel electrophoresis (PFGE) as an epidemiological marker for typing of methicillin-resistant Staphylococcus aureus recovered from KSA
Moussa, I. M.1*, Al-Qahtani, A. A.2, Gassem, M. A.3, Ashgan, M. H.4, Dalia Kadry Ismail5, Ghazy, A. I.3 and Shibl, A. M.6
1Center of Excellence in Biotechnology Research, King Saud University, P. O. Box 2460, Riyadh 11451, King Saudi Arabia.
2Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
3College of Food and Agricultural Sciences, King Saud University, P. O. Box 2460, Riyadh 11451, King Saudi Arabia.
4College of Applied Studies and Community Service, King Saud University, P. O. Box 2460, Riyadh 11451, King Saudi Arabia.
5Kasr Eleini hospital, Cairo University, P. O. Box109, Cairo 11559, Egypt.
6College of Pharmacy, King Saud University, P. O. Box 2460, Riyadh 11451, King Saudi Arabia.
Accepted 18 January, 2019
Abstract
In this study, pulsed field gel electrophoresis (PFGE) was used for genomic DNA finger printings of methicillin-resistant Staphylococcus aureus (MRSA) isolates. Thirty strains of S. aureus collected from major hospital laboratories and public health centers, Riyadh, King Saudi Arabia were tested phenotypically by conventional methods and genotypically by multiplex-PCR for direct detection of S. aureus 16S rRNA and mecA genes. The chromosomal DNA of the isolates was examined by using pulsed-field gel electrophoresis (PFGE) using SmaI. SmaI cut the chromosomal DNA of the examined MRSA into 9 to 13 fragments, moreover, 16 chromosomal digestion patterns were observed out of the 30 examined isolates. The first pulsed field gel electrophoresis (PFGE1) contains 9 strains recovered from soft tissue infections and surgical wound infections. The second one (PFGE 2) contains 4 MRSA isolates, 3 of which were recovered from skin and soft tissue infections, while one was recovered from wound infection. Moreover, there are 3 chromosomal digestion patterns (PFGE 3, 4 and 5), each pattern involved two strains of MRSA which were recovered from surgical wound infections. A dendrogram of percent similarity, revealed three major clusters, the first cluster containing four groups (17 strains). The second cluster contains one group (12 strains), while the third cluster contains only one strain recovered from deep abscess.
Key words: Fingerprinting, Staphylococcus aureus, pulsed field gel electrophoresis, nosocomial infection, multiplex-PCR.