ISSN 2756-3340
African Journal of Infectious Diseases Research ISSN 4729-6836 Vol. 6 (9), pp. 001-006, September, 2019. © International Scholars Journals
Full Length Research Paper
Laboratory evaluation of the entomopathogenic fungi
Zahra Mahdneshin1, Samad Vojoudi*2, Youbert Ghosta1, Mohammad Hassan Safaralizadae1 and Moosa Saber3
1Department of Plant Protection, College of Agriculture, Urmia University, Urmia, Iran.
2Young Researchers club, Marand Branch, Islamic Azad University, Marand, Iran.
3Department of Plant Protection, College of Agriculture, University of Maragheh, Maragheh, Iran.
Accepted 18 April, 2019
Abstract
In this study, the pathogenicity of five Iranian isolates of Beauveria bassiana and Metarhizium anisopliae was evaluated against adults of Callosobruchus maculatus adults by immersion bioassay method at 27 ± 1°C and 60 ± 5% R.H. under laboratory conditions. Cumulative mortality of the cowpea weevil at day 11 after treatment with B. bassiana isolates IRAN 187C, IRAN 429C, IRAN 441C and M. anisopliae isolates IRAN 715C and DEM I001 by conidial suspensions at low and high concentration ranged between 29.4 to 86.2, 9 to 88.3, 21.1 to 96.3, 24.8 to 84.4 and 20.9 to 80.9%, respectively. The LC50 and LT50 values for IRAN 187C, IRAN 429C, IRAN 441C, DEMI001 and IRAN 715C were 1.9×107, 9.8×106, 2.4×107, 2.6×108 and 1.2×108 conidia/ml and 7.8, 6.7, 7.2, 7.7 and 7.8 day, respectively. IRAN 441C of B. bassiana had the highest virulence against adult cowpea weevil because it had lower LC50 and LT 50 and caused the highest mortality (76%) in treatment by suspensions containing 1×108 conidia/ml. B. bassiana had higher virulence than M. anisopliae against adult of cowpea weevil.
Key word: Biological control, immersion bioassay, virulence, cowpea weevil.