ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 19 (1), pp. 001-006, January, 2025. https://internationalscholarsjournals.org/© International Scholars Journals
Full Length Research Paper
Optimizing Blastocyst Formation from Cryopreserved Mouse Two-Cell Embryos: The Role of Fibroblast and Hepatocyte Growth Factors
Fatemeh Ghasemian1, Mahnaz Azarnia2, Abtin Heidarzadeh3, Shervin Ghadarjani4 and Mohammad Hadi Bahadori5*
1Department of Biology, Faculty of Science, Kharazmi (Tarbiat moallem) University, Tehran, Iran.
2Department of Biology, Faculty of Science, Kharazmi (Tarbiat moallem) University, Tehran, Iran.
3Department of Community Medicine, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
4Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
5Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Accepted 5 September, 2022
Abstract
There is great need to improve our understanding of what increases an embryo’s development potential, after vitrification-thawing processes. For this subject, 358 two-cell stage embryos were collected from oviduct of pregnant two-day old mice and vitrified. After thawing, embryos were cultured in Tyrode's (T6) medium supplemented with different doses of fibroblast growth factor (FGF; 0, 10, 20, 50 and 100 ng/ml) and hepatocyte growth factor (HGF; 0, 10, 20, 50 and 100 ng/ml) until the blastocyst stage. To determine quality of blastocysts, blastocysts were stained with hoechst and propidium iodide. After culture for 24 h, 92.65% of treated embryos with 20 ng/ml of FGF had higher (P<0.05) survival rate in comparison to the control group (84%). Blastocyst embryo formation rate were 79.43% (P< 0.01), and 67.46% (P< 0.05) in the treated groups with 20 ng/ml of FGF and 20 ng/ml of HGF, respectively, which were significantly different from the control (56%). In the treatment group with 20 ng/ml of FGF, blastocysts with >64 cells had a significantly higher inner cell mass (ICM) in comparison to the control group (P< 0.01). In conclusion, in this experiment, addition of growth factors in the culture had favorable effects on post-thawed cleavage of vitrified 2-cell embryos and blastocyst quality.
Keywords: Blastocyst quality, growth factors, preimplantation development, vitrification.