ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2756-3707 Vol. 8 (9), pp. 001-005, September, 2021. © International Scholars Journals
Full Length Research Paper
Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro
Li Wang1,2,3, Lizhe An1, Yanping Hu2, Lixin Wei2, and Yi Li2*
1Cold and Arid Regions Environmental and Engineering Research Institute, the Chinese Academy of Science, Lanzhou 730000, China.
2Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau Biology, the Chinese Academy of Science, Xining 810001, China.
3Graduate University of Chinese Academy of Science, Beijing 100049, China.
Accepted 14 July, 2021
Abstract
A procedure for in vitro shoot regeneration was formed from leaves derived from the field grown Swertia chirata and the effects of phytohormones and medium on the regeneration of shoot were tested by an orthogonal experiments. The best result was obtained in the 190-2 medium with 13.32 mM 6-benzylaminopurine and 0.54 mM -naphthaleneacetic acid. The result of orthogonal analysis of variance showed that 6-benzylaminopurine and -naphthaleneacetic acid significantly affected on the shoot regeneration. The propagation rate was 7.75 ± 3.46 when the shoots were cultured on the multiplication medium. The highest rate of shoots was 96.50% on the medium with 5.40 mM -naphthaleneacetic acid. Histological studies showed shoot regeneration took place by somatic embryogenesis.
Key words: Swertia chirata, adventitious shoots, histology, an orthogonal test, leaves.