ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2017
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2017 International Scholars Journals
Full Length Research Paper
Isolation and characterization of Arthrobacter sp. HW08 capable of biodegrading swainsonine
Yan Wang, Yanchun Hu, Jianhua Wang*, Yongtao Yu, Yumin Song, Guodong Yang and Guoxia Geng
Department of Internal Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Shaanxi, China.
Accepted 21 July, 2017
Abstract
The aim of this study was to isolate bacteria capable of degrading swainsonine, an indolizidine alkaloid in poisonous plants of Oxytropis and Astragalus. The bacterium HW08 was isolated and enriched from soil and its physiological and biochemical properties, 16S rRNA gene and the ability of swainsonine-degradation were investigated. The results showed that HW08 was a species of Arthrobacter sp. Under the optimized temperature (30°C) and pH value (7.0), HW08 (OD600 = 0.3) could degrade about 2 mg swainsonine in 5 ml degrading reaction within 4 h. In conclusion, HW08 would be a better potential candidate to be considered for SW-degrading in practice.
Key words: Locoweeds, Arthrobacter; swainsonine, biodegradation.