Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research Vol. 2005

Available online at http://internationalscholarsjournals.org/journal/ajmr

© 2005 International Scholars Journals

Full Length Research Paper

Anther culture response in indica rice and variations in major agronomic characters among the androclones of a scented cultivar, Karnal local

Bidhan Roy, Asit B. Mandal*

Biotechnology Section, Central Agricultural Research Institute, Port Blair 744 105, India.

Accepted 16 August , 2004

Abstract

Effect of organic adjuvants, synthetic plant hormones and diverse carbon sources in influencing anther culture response in five indica rice genotypes (IR 72, Mansarovar, Taraori Basmati, Pusa Basmati and Karnal local 95) were assessed. Androgenic callus induction as well as green plantlet regeneration was more when callus was induced on N6 fortified with 100 mg L^-1 YE. However, coconut water (5 and 10% concentrations from Pink Dwarf variety) encouraged green plantlet regeneration only in Karnal local 95. High callus induction was observed with synthetic hormones in comparison to the control set. However, green plantlet regeneration was observed when callus induction medium (CIM) was supplemented with 2, 4-D and IBA in Taraori Basmati and Karnal local 95. Among the varieties Pusa Basmati and Karnal local 95 showed better callus inductions on N6 supplemented with 6% maltose. Maximum callus induction was observed in Pusa Basmati and Karnal local 95 when 6% sucrose or maltose were used as carbon source. Total plantlet regeneration was cent percent in Taraori Basmati when CIM was supplemented with maltose (6%). Maximum green plants were obtained when CIM was added with maltose (6%) for Karnal local 95. Plantlet regeneration in the present study was found to be very low. Androgenic plantlets derived from the scented indica rice, Karnal local 95, were evaluated under field condition to assess variability among segregating A₂ generation. Overall mean values in respect of some major agronomic characters viz. plant height, panicle length, number of filled grains per panicle, spikelet sterility (%) and grain yield per plant were reduced except number of panicles per plant. High CV was observed for filled grains per panicle, grains yield per plant and number of panicles per plant suggesting the existence of high variation among the androclones for those characters. Positive selection deems to be improvising those characters. The frequency distribution for number of panicles per plant and panicle length varied largely among the androclones in both direction of the parental mean. Whereas, all the androclones were found to be shorter than the parent and low yielder too. This study elucidates that the genetic modulation through exploitation of androclonal variation is a feasible proposition in scented indica rice.

Key words: Scented rice, anther culture, androclonal variation, genetic improvement.