ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN: 2169-3048 Vol. 5 (3), pp. 670-674, March, 2016. © International Scholars Journals
Full Length Research Paper
Determination of callus induction of the artichoke and optimization of callus producing culture
Pierre Reza Shan*, Mir-Hossein Banisadr Nasr and Abolhassan Malek-Yonan
University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
E-mail: [email protected]
Accepted 13 February, 2016
Abstract
In the present research, the effects of different culture media and hormone concentrations on the callus production of different organs of the artichoke were investigated. The obtained seedlings were transferred to half-strength MS medium. Only non-contaminated seedlings were used for preparation of root, leaf and petiole explants. The SH, MS and B5 media containing 0.8% agar, 3% sucrose and 0, 0.5, 0.75 and 1 (mg/l) 2,4-D were used for callus induction. The samples were kept in 25±2°C and at dark conditions. No callus formation was observed in hormone free medium. The experiment was performed based on a completely randomized plan and in 3 replications. According to the results, it was found that the values of callus formation and callus dry mass (dry weight) were statistically different (α<0.05) among various media. So, the highest callus formation was observed in leaf explants which was planted in B5 medium containing 1 (mg/l) 2,4-D. Callus dry mass in the petiole explant in the B5 medium with supplemented 0.5 (mg/l) concentration of hormone was significantly higher than that of the other media.
Key words: Artichoke, explant, 2,4-D, culture media, callus induction.