Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research Vol. 2005

Available online at http://internationalscholarsjournals.org/journal/ajmr

© 2005 International Scholars Journals

Full Length Research Paper

Callus proliferation and somatic embryogenesis in cotton (Gossypium hirsutum L.)

Ikram-ul-Haq

Researcher Plant Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), P. O. Box 577, Jhang Road, Faisalabad, Pakistan. E-Mail: [email protected].

Accepted 14 July, 2004

Abstract

Somatic embryogenesis and plant regeneration are fundamental to tissue culture biotechnology in cotton (Gossypium hirsutum L.) cv. Coker 312. Callus proliferation was considered best on MS_1a (2.0 mg/L NAA; 0.1 mg/L ZT; 0.1 mg/L KT) when 6 weeks old callus was cultured from MS_1b (0.1 mg/L 2, 4- D; 0.5 mg/L KT) medium, there is no need to select embryogenic calli for somatic embryogenesis, as all of them were converted to somatic embryos. NH₄NO₃ play an important role in differentiation of callus into somatic embryos but is lethal for embryos just after two weeks. However, KNO ₃ is less efficient for somatic embryo induction but is best for embryo maturation. By this procedure 56.51% cotyledenary embryos were developed within 5 weeks. Of that, 82.05% cotyledenary embryos were developed not only into normal plantlets, but rooted simultaneously when cultured on MS (with 0.05 mg/L GA₃) medium. A complete plant of Cocker-312 could be regenerated through somatic embryogenesis within 4 to 5 months.

Key words: Gossypium hirsutum L,plant regeneration, Coker 312, callus induction, somatic embryogenesis, in vitro regeneration.