ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (7), pp. 001-007, July, 2018. © International Scholars Journals
Full Length Research Paper
Antiviral activity and mode of action of Dianthus caryophyllus L. and Lupinus termes L. seed extracts against in vitro herpes simplex and hepatitis A viruses infection
Ahmed B. Barakat1*, Sahar A. Shoman1, N. Dina2 and Omar R. Alfarouk1
1Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt.
2Department of water pollution, National Research Center, Dokki, Egypt.
Accepted 28 January, 2018
Abstract
Crude extracts of sixteen seeds belonging to different plant species were tested for their antiviral activity against herpes simplex virus-1 (HSV-1) and hepatitis A virus-27 (HAV-27). Non-toxic concentration (20 µg/ml) of Dianthus caryophyllus and Lupinus termes seed extracts to both Vero and HepG2 cells showed potent antiviral activity against HSV-1 and HAV-27 using plaque infectivity count assay. The mechanism of action D. caryophyllus revealed its virucidal activity against HSV-1 and HAV-27 as 92.3 and 92.6%, respectively, while, the virucidal activity of L. termes was observed only against HAV-27 giving 93.7% of inhibition. No effect was detected for both extracts on adsorption or on the stages of virus replication. A comparison has been done between the antiviral activity of two therapeutic drugs (Acyclovir and Amentadine used as controls for HSV-1 and HAV-MBB, respectively) and the two tested seed extracts. The results revealed that these seed extracts were more efficient in their inhibitory activity than synthetic chemical drugs against the same viruses. This may open the way to give more attention to use the natural botanical origin in treatmenting viral infection with or without therapeutic agents to obtain better recovery with least side effects.
Key words: Antiviral seed extract, herpes virus infection, hepatitis virus infection, amantadine, acyclovir.