ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-005, February, 2019. © International Scholars Journals
Full Length Research Paper
Identification and characterization of 16 recent Chinese isolates of bovine viral diarrhea virus genotype 2 (BVDV-2)
Tao J., Zhu l. Q., Lin Y. Q. and Zhu G. Q.*
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Accepted 09 January, 2019
Abstract
Eighty calf blood samples were collected from different areas of China and were examined. Sixteen non cytopathic isolates of bovine viral diarrhea virus genotype 2 (BVDV-2) were isolated by specific BVDV-2-directed RT-PCR and sandwich enzyme-linked immunosorbent assay (ELISA). The results were verified by the indirect immunofluorescence assay and the ultra-thin sections. Genes of 5'-UTR, E2 and Npro from all the 16 field isolates were sequenced. The sequence identities at the nucleotide and amino acid levels were higher than 99.0%. The phylogenetic analysis revealed that the BVDV-2 USA strain, AzSpin, had the highest sequence homology with each of the Chinese isolates.
Key words: Bovine viral diarrhea virus genotype 2 (BVDV-2), isolation and characterization, phylogenetic analysis.