ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 10 (6), pp. 001-005, June, 2016. © International Scholars Journals
Full Length Research Paper
Characterization of quinapyramine (Trypacide®) drug-resistant Trypansoma evansi
Intisar E. El Rayah1* and Khitma H. El Malik2
1Trypanosomosis Unit, Tropical Medicine Research Institute (TMRI), P.O.BOX 1304, Khartoum 11111, Sudan.
2Faculty of Veterinary Science, University of Khartoum, P.O Box 32, Khartoum North, Sudan.
Accepted 30 March, 2016
Abstract
Molecular karyotyping by pulsed field gel electrophoresis was used to characterize Trypanosoma evansi isolates. Ten T. evansi isolates from camels were collected in Eastern and Western Sudan. Isolates from Eastern Sudan which were kept under continuous prophylactic treatment with quinapyramine (Trypacide®), were found to bear a single pattern and belonged to one karyotype group. From Western Sudan where trypanosomosis management was done by individual treatment of proven parasitaemic cases, isolates with diverse karyotype patterns were obtained. This study concluded that the occurrence of karyotype homogeneity amongst T. evansi isolates from field situations where anti-trypanosomal compounds have been used may infer the existence of drug-resistance.
Key words: Trypanosoma evansi, drug resistance, Trypacide®, karyotyping, Sudan.