Advanced Journal of Microbiology Research

Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 18 (8), pp. 001-004, August, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals

Full Length Research Paper

Direct Detection of Salmonella InvA, SpiC, and SipC in Clinical Samples Using Dot Blot Hybridization

Hang’ombe Bernard Mudenda¹*, Ulaya William¹, Mwansa James C. L.², Mubita Charles¹, Isogai Nayuta³, Mulenga Evans¹, Moonga Ladslav¹, Isogai Hiroshi⁴ and Isogai Emiko⁵

¹School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia.
²University Teaching Hospital, Department of Pathology and Microbiology, Lusaka, Zambia.
³Department of Agro-bioscience, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
⁴Animal Research Center, Sapporo Medical University, 060-8556, South 1 West 17, Chuoh-ku, Sapporo, Japan.
⁵Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiya-Machi Aoba-ku, Sendai 981-8555, Japan.

Accepted 28 March, 2024

Abstract

Pathogenesis of Salmonella depends upon a large number of factors controlled by an array of genes that synergise into actual virulence. The goal of this study was to detect Salmonella invA, spiC and sipC directly from clinical specimens, using the dot blot hybridization assay. We detected invA , spiC and sipC as a one combination from 4.5% (95% CI: 2.21 to 8.64) human feacal and 35.2% (95% CI: 26.4 to 45.0) poultry samples after enrichment. Furthermore the dot blot method had a higher sensitivity than routine culture, before and after enrichment. These results indicate that dot blot hybridization may be used to directly detect Salmonella invA, spiC and sipC in clinical samples.

Key words: Salmonella, dot-blot hybridization, spiC, sipC, invA, clinical-samples.