ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
Isolation and characterization of Acinetobacter sp. ND12 capable of degrading nicotine
Hongjuan Li1, Yanqing Duan2, Guanghui Ma1, Liping Lei3, Ke-Qin Zhang1 and Jinkui Yang1*
1Laboratory for Conservation and Utilization of Bio-Resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, P. R., China.
2Technology Centre of Hongyun Honghe Tobacco (Group) Co, Ltd., Kunming 650202, P. R., China.
3Tobacco Agricultural Science Institute of Yunnan Province, Yuxi 653100, P. R., China.
Accepted 19 January, 2019
Abstract
A bacterium strain ND12, was isolated from the tobacco plantation soil and identified as Acinetobacter sp. based on its morphology, physiology, and 16S rRNA gene sequence. The isolate could utilize nicotine as the sole source of carbon and nitrogen, and completely degrade 1.0 g/L nicotine within 14 h at 28°C and pH 6.0. The optimum nicotine concentration for the growth of strain ND12 was 2.5 g/L. Strain ND12 grew at a broader pH range than other reported nicotine-degrading bacteria and showed higher nicotine-degrading activity than those bacteria at acidic environment (pH 4.0). The resting cells of Acinetobacter sp. ND12 could decompose 90% of the nicotine in upper tobacco leaves after treating for 11 h. Moreover, the nicotine metabolites of strain ND12 were analyzed by high performance liquid chromatography (HPLC) and compared with Arthrobacter sp. HJ2-3 and Pseudomonas sp. Nic22. Our results suggested that strain ND12 could be useful for reducing nicotine concentration in nicotine-polluted environments and tobacco wastes.
Key words: Nicotine, biodegradation, Acinetobacter sp. ND12, resting cells, tobacco leaves.