ISSN 2756-3685
International Journal of Enology and Viticulture Vol. 5 (7), pp. 001-011, July, 2018. © International Scholars Journals
Full Length Research Paper
In vitro investigation of the hypoglycemic activity of yeasts using models of rat epididymal adipocyte and differentiated mouse 3T3-L1 adipocyte
Chien-Hui Wu1,2, Hong-Ting Lin1, Guan-James Wu3, Sheng-Hong Wang1 and Guo-Jane Tsai1,*
1Department of Food Science, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan, ROC.
2Department of Food and Beverage Management, Mackay Medicine, Nursing and Management College, No. 92, Shengjing Road, Beitou District, Taipei City 11260, Taiwan, ROC.
3Department of Food Science, National Penghu University of Science and Technology, No. 300, Liu-Ho Road, Makung, Penghu county 880, Taiwan ROC.
Accepted 10 May, 2018
Abstract
The differentiated mouse 3T3-L1 adipocytes (3T3-L1 model) were used in studying glucose metabolisms without the need for feeding (Sprague-Dawley, SD model) the rat prior to hypoglycemic activity evaluation. Both models were adopted to evaluate the hypoglycemic activities of 58 yeast strains isolated from various sources (grape, vine yard soil, winery soil). Among the 58 tested yeast isolates, strain 54 (Saccharomyces pastorianus no. 54) which showed the highest hypoglycemic activity was chosen to be the test strain. The optimal insulin concentration used in these 2 models (SD and 3T3-L1) for measuring the hypoglycemic activity of hypoglycemic yeast extract (HGYE) was 10 nM. The range of linear relation in the dose-response curve was 0-1000 g/ml for SD model, and 0-250 g/ml for 3T3-L1 model. The linear coefficient was 0.8611. The radioactive labeled 2-[1-14C]-Deoxy-D-Glucose was also used to confirm cytoplasmic glucose uptake by 3T3-L1 adipocytes. Comparing both the results of insulin effect and dose response of HGYE by both models, it was concluded that the 3T3-L1 model can serve as a rapid and reliable assay model for in vitro evaluation of hypoglycemic activity of yeast.
Key words: 3T3-L1 adipocytes, Sprague-Dawley (SD) rat, epididymal adipocytes, hypoglycemic activity, yeast.