ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2756-3707 Vol. 6 (4), pp. 001-004, April, 2019. © International Scholars Journals
Full Length Research Paper
Autofluorescence of routinely hematoxylin and eosin-stained sections without exogenous markers
Deeb S.*, Nesr K. H., Mahdy E., Badawey M. and Badei M.
Department of Pathology, Faculty of Veterinary Medicine, University of Beni Suef, Egypt.
Accepted 16 January, 2019
Abstract
Hematoxylin and eosin stained paraffin section was examined by fluorescence microscopy to study the pattern and distribution of fluorescence. Autofluorescence was sensitive and specific for detection of elastic and collagen fibers. It was concluded that analytical morphological techniques based on autofluorescence can obtain information about morphological and pathological state of tissue and cells.
Key words: Histology, pathology, fluorescence microscopy.