ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 2 (6), pp. 180-189, June, 2015. © International Scholars Journals
Review
Thin Cell Layer technology and their application in ornamental plant micropropagation
Esaki Toshiki
Department of Agronomy, Faculty of Agriculture, University, Kinjo University. E-mail: esaki.toshili@ kinjo.ac.jp
Accepted 02 August, 2014
Abstract
Thin cell layer (TCL) technology originated almost 30 years ago with the controlled development of flowers, roots, shoots and somatic embryos on tobacco pedicel longitudinal TCLs. Since then TCLs have been successfully used in the micropropagation of many ornamental plant species whose previous in vitro regeneration was not successful using conventional methods. This review examines the fundamentals behind TCLs, and their application in ornamental plant micropropagation and transformation.
Key words: Chrysanthemum, lily, somatic embryogenesis, thin cell layer, tobacco, transformation.